摘要
目的:探讨自噬相关基因宫颈鳞癌组织中微管相关蛋白1轻链3(LC3)的蛋白表达及与肿瘤发生、发展的关系。方法:采用免疫组化的方法检测122例宫颈鳞状细胞癌,35例宫颈上皮内瘤变(CIN)Ⅱ～Ⅲ,31例正常宫颈组织LC3蛋白的表达。结果:宫颈鳞癌、CIN组织和正常宫颈组织组织中LC3阳性率分别为51.6%、71.4%和83.9%,3组中LC3阳性表达呈下降趋势,χ2=20.837,P=0.000。LC3与肿瘤分化(χ2=16.678,P=0.000)、复发(χ2=4.817,P=0.028)相关。鳞状细胞癌抗原(SccAg,P=0.042)、肿瘤分化(P=0.010)、浸润深度(P=0.048)、淋巴结转移(P=0.001)、手术切缘(P=0.015)和LC3(P=0.025)表达是无进展生存时间(PFS)的危险因素;肿瘤分化(P=0.025)、淋巴结转移(P=0.001)是影响总生存时间(OS)的危险因素。多因素分析显示,淋巴结转移是PFS(P=0.015)和OS(P=0.002)的独立预后因素。结论:宫颈鳞癌组织LC3表达低于正常宫颈和CIN组织的表达,提示LC3在宫颈癌的发生、发展中可能起一定作用。
OBJECTIVE： To study the role of microtubule-associated protein 1 light chain 3（LC3） in the genesis and progression of cervical cancer by detecting LC3 expression in different cervical tissues.METHODS： Immunohistochemistry technique was used to detect the expression of LC3 gene in specimens of 122 cervical squamous cell carcinoma,35 cervical intraepithelial neoplasia（CIN）,and 31 normal cervical tissues.RESULTS： The positive expression rate of LC3 in cervical squamous cell carcinoma,CIN and normal cervix was 51.6%,71.4% and 83.9% respectively.The LC3 positive expression decreased gradually as the severity of the lesion progressed from normal cervical epithelium to infiltrating carcinoma significant（χ2=20.837,P=0.000）.The expression of LC3 was associated with tumor differentiation（χ2=16.678,P=0.000） and recurrene（χ2=4.817,P=0.028）.Univariate analysis of clinicopathlogical factors revealed that Squamous cell carcinoma antigen（SccAg,P=0.042）,tumor differentiation（P=0.010）,depth of invasion（P=0.048）,pelvic lymphnode metastasis（P=0.001）,incisal edge of operation（P=0.015） and LC3（P=0.025）were risk factors for progressive-free survial（PFS）;while tumor differentiation（P=0.025）and pelvic lymphnode metastasis（P=0.001）were risk factors for overall survival（OS）.Multivariate analysis showed that pelvic lymphnode metastasis were the independent prognostic factors for progressive-free survial（PFS,P=0.015） and overall survival（OS,P=0.002）.CONCLUSION： The positive expression of LC3 in cervical squamous cell carcinoma is significantly lower than that in nomal cervical tissue and CIN,which implies that it may play a role in carcinogenesis and development of cervical squamous cell carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2011年第16期1247-1251,共5页
Chinese Journal of Cancer Prevention and Treatment
