摘要
目的观察嗜酸性粒细胞阳离子蛋白(ECP)及嗜酸性粒细胞趋化因子(eotaxin)在鼻内镜术后鼻腔黏膜转归过程中表达的变化,并分析两者的相关性,探讨嗜酸性粒细胞聚集活化对鼻内镜术后鼻腔黏膜转归的影响。方法采用酶免疫荧光分析技术检测ECP,采用Western-blot技术检测eotaxin,分析ECP及eotaxin在慢性鼻-鼻窦炎患者行鼻内镜术后鼻腔黏膜转归过程中的表达变化。结果 ECP及eotaxin蛋白在正常鼻黏膜中少量表达,术后随时间呈现如下变化规律:①术后1～2周较术前明显降低;②术后4～6周达高峰,基本恢复至术前水平;③术后10～12周回落,接近于术后1～2周水平。④eotaxin和ECP在鼻内镜术后鼻腔黏膜转归过程中呈正相关关系,相关系数为0.719。结论 eotaxin、ECP在鼻内镜术后鼻腔黏膜的表达动态变化与鼻腔病变的转归过程相对应。
Objective To observe the expression of eotaxin and ECP during different phrases of process of mucosa in sinus cavity after endoscopic sinus surgery,and investigate the roles of eosinophils in the pathogenesis of CRS and the clinical mucosa recovery.Methods The expression of eotaxin and ECP during different phrase of process of mucosa in sinus cavity wre determined by using Western-blot and enzyme-fluoroimmunoassay respectively.The datas were analyzed with ANOVA and linear correlation with SPSS 11.0 software.Results From the first to second week after surgery,there was a significant decrease in secretion of eotaxin and ECP.The secretion of eotaxin and ECP rose back at the 4 to 6 week after operation,and tended to the level of the first to second at the 10 to 12 week after operation.Eotaxin was positively correlated to the secretion of ECP during the mucosa recovery in operative cavity（r=0.719,P〈0.01）.Conclusion Dynamic expressions of eotaxin and ECP in the operative cavity mucosa are correspondence to the clinical mucosa recovery.Our data demonstrate the gathering and acting of eosinophils related to the edema and polyoid hypertension closely.
出处
《东南国防医药》
2011年第5期419-421,共3页
Military Medical Journal of Southeast China
