摘要
参考1型鸭肝炎病毒基因组序列,设计7条引物,用RT-PCR方法扩增出覆盖整个病毒基因组三个忠实性片段,并按顺序组装进载体pBR322中,获得全长cDNA克隆。测序结果表明,该克隆与母本毒序列同源性达99.6%,并且5'端的T7启动子和3'端的MluⅠ线性化位点均成功引入。
Seven primers were synthetized according to genome sequence of duck hepatitis virus （DHV）typel, and three fidelity were amplified by RT-PCR, and these DNA fragments covering the full genome of DHV typel fragments were inserted into pBR322 vector according to the genome sequence, then gained the full-length cDNA clone.Sequence homology is 99.6% with the clone and female parent virus, and T7 promotor and Mlu I site are respectively added to genome 5＇ end and 3＇ end.
出处
《家禽科学》
2011年第10期10-13,共4页
Poultry Science
基金
山东省自然科学基金项目资助(Y2008D55)
关键词
1型鸭肝炎病毒
RT—PCR
全长CDNA克隆
duck hepatitis virus （DHV）typel
reverse transcription PCR
the full-length clone. cDNA
