摘要
研究了从鼓槌石斛中分离得到的低分子量天然化合物毛兰素在体外对人肝癌Huh7细胞的增殖抑制作用,并探讨其诱导细胞凋亡的分子机制.实验结果显示:毛兰素能显著抑制肝癌Huh7细胞的增殖,且随着药物浓度与时间增加其抑制率呈明显的剂量时间效应,其48 h半数抑制浓度IC50为37.4 nmol/L;毛兰素能诱导人肝癌Huh7细胞凋亡并使其细胞周期阻滞于G2-M期;分子机制研究显示毛兰素可抑制Akt激酶活性、下调Mcl-1蛋白表达以及激活PARP活性从而导致其对Huh7癌细胞的增殖抑制作用.以上结果初步表明毛兰素对肝癌防治具有潜在药用价值.
Erianin,a low molecular-weight natural product isolated from Dendrobium chrysotoxum Lind.,is often used as an anti-pyretic and an analgesic in traditional Chinese medicine.Herein,this study evaluated the erianin＇s effect on cell proliferation,apoptosis and cell cycle progression of hepatocellular carcinoma（HCC） Huh7 cancer cells especially.The results in vitro demonstrated that erianin significantly suppressed proliferation of Huh7 cancer cells in a dose-and time-dependent manner which was associated with induced apoptosis and cell cycle G2-M arrest,and the IC50 value was 37.4 nmol/L after 48 h exposure to erianin.Mechanistically,erianin could inhibit Akt activation,down-regulate the expression of Mcl-1 protein,and activate the cleavage of PARP in a dose-and time-dependent manner,leading to marked inhibitory effect of Huh7 cells.These findings suggest that erianin may be a promising antitumor drug for prevention and treatment of HCC.Fig 5,Ref 17
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第5期662-665,共4页
Chinese Journal of Applied and Environmental Biology
基金
中国科学院知识创新工程领域前沿项目(No.KSCX2-EW-J-22)
四川省国际合作计划(No.2011HH0012)
国家自然科学基金项目(No.81072662)
中国科学院"西部之光"人才培养计划重点项目资助~~
