摘要
从浙江华海药业污水处理系统中分离得到一株硫氧化细菌T3,基于形态特征、生理生化、16S rRNA基因序列系统学分析和Biolog鉴定系统分析,鉴定该菌株为根瘤菌属.摇瓶实验结果表明,T3生物降解最适生长温度为30℃,最适pH值为8.0,外加氯化铵、碳源对菌株生长及硫化钠降解有促进作用,驯化后的硫氧化细菌对硫化钠有很强的耐受能力,最优生长条件下,2 d内菌株T3能将400 mg/L以下浓度的硫化钠降解彻底,是一株有应用前景的硫氧化细菌.通过测定代谢过程中各种物质的含量,确定该菌株对硫化钠的去除机理为S2-→S2O32-/S0→SO32-→SO42-.
A sulfide-oxidizing bacterium T3 was isolated from the wastewater treatment system in Zhejiang Huahai Pharmaceutical Factory of China.The strain was identified as Rhizobium sp.based on the morphological,physiological-biochemical characteristics,sequence analysis of 16S rRNA and the Biolog Identification System.The optimal temperature and pH for T3 biodegradation in the shaking flasks were 30 ℃ and 8.0,respectively.With the additional ammonium chloride and carbon sources,the degradation ability was enhanced.The acclimated bacteria had strong tolerance to sodium sulfide,and could completely degrade sodium sulfide（below 400 mg/L） within two days under the optimal growth condition,which showed their great application prospect.During the course of sodium sulfide oxidation by the strain,various intermediate metabolites were analyzed.The results indicated that the removal mechanism was followed by S2-→S2O32-/S0→SO32-→SO42-.Fig 8,Tab 1,Ref 18
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第5期706-710,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家"863"计划项目(No.2009AA062603)资助~~
关键词
硫氧化细菌
根瘤菌属
硫化钠
生物降解
污水处理
sulfide-oxidizing bacterium
Rhizobium
sodium sulfide
biodegradation
wastewater treatment
