摘要
目的观察急性心肌梗死(AMI)患者经皮冠状动脉腔内成形术(PTCA)前后外周血中细胞黏附分子(CAMs)的动态表达水平,探讨其与再灌注损伤的关系。方法采用流式细胞术和酶联免疫法分别测定79例AMI患者PTCA前,术后30min、2、6、12、24、72h的血小板颗粒糖蛋白(CD62p)、中性粒细胞表面黏附分子(CD11b)、可溶性细胞间黏附分子(sICAM-1)、可溶性血管黏附分子(sVCAM-1)的表达水平,36例冠状动脉造影正常者为对照组。结果 AMI患者术前CD62p、CD11b、sICAM-1和sV-CAM-1)的表达水平均明显高于对照组(P<0.01),术后30min AMI组CD62p水平与术前相比明显下降(P<0.05),但随之增高,至12h达高峰,72h降为术前水平,CD11b术后水平持续增高,12h达高峰,72h仍高于术前(P<0.05),而AMI组sICAM-1和sVCAM-1的术后表达水平持续增高,但无明显峰值。直线相关分析,AMI组术后12hCD62p水平与CD11b的表达水平呈正相关(r=0.673,P<0.01),而sICAM-1和sVCAM-1的术后24、72h表达水平呈正相关(r=0.562,r2=0.557,P<0.05)。结论 PTCA术可能导致AMI患者急性炎症反应,CD62p、CD11b、sICAM-1和sVCAM-1的异常表达增高与再灌注损伤有关。
Objective To observe dynamic changes of cell adhesion molecules（CAMs） in patients with acute myocardial infare- tion（AMI） after percutaneous transluminal coronary angioplasty（PTCA）, and to explore the relationship between the CAMs and myocardial ischemia-reperfusion injury（MIRI）. Methods The level of CD62p and CDllb in 79 cases of patients were determined by flow eytometry（FCW）, and the level of slCAM-1 and sVCAM-1 were determined by ELISA, before therapy and 30 mins, 2, 6, 12, 24, and 72 hours after PTCA. 39 cases of normal controls demonstrated by coronary angiography were also determined. Results The levels of CD62p, CDllb, sICAM-1, and sVCAM 1 in the patients before therapy were significantly higher than that in controls（P〈0. 01）. The levels of CD62p at 30 mins after PTCA were significantly lower than that in patients with AMI before PTCA （P〈0.05）, and then increased, arrived at the peak at 12 h after PCTA. The levels of CD62p at 72h were not significantly different from that in the patients before PTCA （P〉0.05）. The levels of CDllb after PTCA were increased, reached peak at 12 h, and were still high at 72 h. The levels of sICAM-1 and sVCAM-1 after PTCA were continued increasing, but no peak. The levels of CD62p were correlated with CDllb after PTCA at 12h （r=0. 673,P〈0.01）, and the levels of slCAM-1 were correlated with sVCAM-1 after PCTA at 24h and 72h （r1 =0. 562 ,r2 =0. 557,P〈0.05）. Conclusion PTCA may lead to acute inflammatory response in patients with AMI, and the high expressions of CD62p, CDllb, sICAM-1, and sVCAM-1 were positively related to MIRL .
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第30期3048-3050,共3页
Chongqing medicine
关键词
心肌梗死
急性
血管成形术
经腔经皮冠状动脉
再灌注
损伤
细胞黏附分子
myocardial infarction
angioplasty, transluminal percutanrous coronary
reperfusion, injury
cell adhesion molecules
