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基质辅助激光解吸电离飞行时间质谱在临床微生物领域的应用进展 被引量：6

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摘要 在临床微生物实验室,革兰染色、菌落形态、显微镜检查、选择培养基的生长特点、各种手工或自动的生化试验是细菌、酵母、真菌的主要鉴定方法。这些方法依赖细菌生产代谢过程,需要长时间的孵育,一般需要24～48h,不能满足临床对感染患者的快速诊断和适当的治疗。分子生物学方法、细菌核糖体（16SrRNA）基因序列分析、实时荧光定量PCR检测特定的基因被认为是替代方法，但这些技术复杂、耗费高，不适用于临床微生物实验室的检测工作。
作者 颜英俊 汤一苇
机构地区 四川省医学科学院/四川省人民医院 美国范德堡大学医学中心
出处 《国际检验医学杂志》 CAS 2011年第17期1969-1972,共4页 International Journal of Laboratory Medicine
基金 美国国立卫生研究院基金资助项目(UL1-RR024975)
关键词 细菌 真菌 病毒 基质辅助激光解吸电离飞行时间质谱 应用
分类号 R446.5 [医药卫生—诊断学]
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参考文献37

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同被引文献41

  • 1马庆伟,程肖蕊.MALDI-TOF MS引领分子诊断新时代[J].生物技术世界,2006(5):66-68. 被引量：2
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  • 6van Veen SQ, Claas EC, Kuijper EJ. High-throughput identifica- tion of bacteria and yeast by matrix-assisted laser desorption ioniza- tion-time of flight mass spectrometry in conventional medical micro- biology laboratories. J Clin Microbiol, 2010, 48(3) : 900-907.
  • 7Bizzini A, Durussel C, Bille J, et al. Performance of matrix-assis- ted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical mi- crobiology laboratory. J Clin Microbiol, 2010, 48 ( 5 ) : 1549- 1554.
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国际检验医学杂志
2011年 第17期

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