摘要
目的探讨环孢素A(CsA)联合牙龈卟啉单胞菌脂多糖(Pg-LPS)对人牙周膜成纤维细胞(hPDLFs)增殖的影响。方法改良组织块法培养hPDLFs,分别用CsA(10、100、200、400ng/ml),Pg-LPS(10、100、1000ng/ml),CsA(100ng/ml)加Pg-LPS(100ng/ml),CsA(100ng/ml)加Pg-LPS(1000ng/ml)作用于生长良好的第3～5代细胞,MTT法测其药物作用用下的增殖情况。结果细胞经上述浓度CsA或Pg-LPS作用后形态无明显变化,CsA在100ng/ml浓度情况下,促细胞增殖作用最明显;高浓度Pg-LPS(1000ng/ml)显著抑制细胞增殖,100ng/mlCsA能够显著降低Pg-LPS(1000ng/ml)对细胞增殖的抑制效应;CsA(100ng/ml)与Pg-LPS(100ng/ml)联合作用于细胞时,对细胞的增殖无明显影响。结论在一定的浓度下,CsA促PDLF增殖作用明显;CsA能对抗脂多糖(LPS)抑制细胞增殖的效应。
Objective To evaluate the effects of cyclosporine A （CsA） and Porphyromonas gingivalis-lipopolysaccharide （Pg-LPS） on the proliferation of human periodontal ligament fibroblasts （hPDLFs） in vitro.Methods hPDLFs were cultured by using modified tissue block culture method.Cells of the 3rd and 5th passage were then incubated with CsA （10 ng/ml,100 ng/mL,200 ng/ml,400 ng/ml）,Pg-LPS （10 ng/ml,100 ng/ml,1000 ng/ml）,CsA （100 ng/ml）＋ Pg-LPS （100 ng/ml） and CsA （100 ng/ml）＋ Pg-LPS （1000 ng/ml）.Cell proliferation was assessed with MTT assay.Results No significant alterations of the cellular morphology could be observed after drug exposure.100 ng/ml CsA enhanced hPDLFs proliferation;1000 ng/ml Pg-LPS inhibited hPDLFs proliferation,whereas 100 ng/ml CsA significantly decreased such inhibitory effect;hPDLFs proliferation was not significantly affected when incubated with CsA（100 ng/ml） plus Pg-LPS（100 ng/ml）.Conclusion At definite concentration,CsA could enhance hPDLFs proliferation,and decrease the inhibitory effect of Pg-LPS of high concentration.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2011年第5期11-15,共5页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
福建省科技重点项目(2009Y0019)
福建医科大学苗圃科研基金(2010MP016)
福建省科技资助省属高校课题(2008F5023)
关键词
环孢素A
脂多糖
牙周膜成纤维细胞
Cyclosporine A
Porphyromonas gingivalis-lipopolysaccharide
Periodontal ligament fibroblast
