摘要
分别将离子交换树脂法和大孔吸附树脂法用于对芸豆蛋白酶解物(KBPHs)的脱盐工艺,并对其进行比较研究,分析脱盐前后KBPHs的理化成分和DPPH.清除活性。结果表明,大孔吸附树脂对KBPHs的脱盐效果优于离子交换树脂。静态吸附和解吸实验表明,H103树脂的吸附性能优于其他3种树脂。动态脱盐的最佳条件为:pH4.5、浓度45.5mg/mL的KBPHs液以1BV/h的流速上样、1BV/h的流速水洗、2mL/min的80%乙醇解吸。该条件下,H103大孔树脂对KBPHs的脱盐率为87.31%,回收率达82.37%。脱盐后KBPHs中灰分含量仅为1.81%,DPPH.清除活性的HSC50值为0.53mg/mL。
In order to obtain optimal technological processing for desalinization of kidney bean protein hydrolysates（KBPHs）,comparison between ion exchange resin（IER）and macroporous adsorption resin（MAR）was conducted and the optimal conditions of the two methods was determined.Physicochemical compositions and DPPH· scavenging activities of KBPHs using both desalinization methods were also compared.The results showed that the desalinization effect of MAR was better than that of IER.The experiments of static adsorption and desorption showed that MAR H103 had the highest adsorption-desorption capability to KBPHs among 4 resins.In further study,the optimal desalination conditions of KBPHs with the resin H103 were confirmed as follows：pH of KBPHs 4.5,concentration of KBPHs 45.5mg/mL,flow rate of water washing 1BV/h,and desorption rate of 80% ethanol 2.0mL/min.Under these conditions,the desaltion rate reached about 87.31%,and the recovery of KBPHs was about 82.37%.In the optimization of condition,KBPHs had a remarkable DPPH· scavenging activities,HSC50 was 0.53mg/mL.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第11期309-311,314,共4页
Science and Technology of Food Industry
基金
2009年国家大学生创新计划项目
甘肃省青年科技基金(1107RJYA065)
关键词
芸豆蛋白酶解物
离子交换树脂
大孔吸附树脂
脱盐
DPPH·清除活性
kidney bean protein hydrolysate（KBPH）
ion exchange resin
macroporous adsorption resin
desalination
DPPH· scavenging activities
