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Research of vitamin E succinate combined with paclitaxel on the apoptosis of Her-2 over-expressing breast cancer cells

维生素E琥珀酸酯联合紫杉醇对Her-2过表达乳腺癌细胞的诱导凋亡研究(英文)
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摘要 Objective: The aim of this study was to detect apoptosis rates of Her-2 overexpression breast cancer cells, which were administrated with vitamin E succinate (VES) combined with paclitaxel at different dosages, or administrated alone; to discuss the mechanism of their actions. Methods: Using immunohistochemical method to detect Her-2 expression of MDA- MB-453 cells. Using TUNEL assay to detect apoptosis rates of MDA-MB-453 ceils, with the concentrations at 10, 20 mg/L of VES and 50, 100 nmol/L of paclitaxel, and also combined together for 24 or 48 h. Then compared apoptosis action of various combinations. Results: The expression rate of 95% Her-2 was interval (63.32%, 69.60%); VES and paclitaxel both induced apoptosis of MDA-MB-453 cells, and it is dose to time dependence. It was strongest in apoptosis at 10 mg/L VES and 100 nmol/L paclitaxel in MDA-MB-453 cells 48 h later. Conclusion: VES and paclitaxel both induced apoptosis of MDA-MB-453 cells. It is stronger when the two drugs are administrated together. The mechanism is probably related to reduction of bcl-2 expression, so as to be more sensitive to paclitaxel. Synergistic effect is also possible for the two drugs influence tumor cells in different growing phases.
机构地区 Department of Oncology
出处 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第11期662-665,共4页 中德临床肿瘤学杂志(英文版)
基金 Supported by a grant from the Educational Department of Province Heilonjiang (No. 11521185)
关键词 vitmian E succinate (VES) PACLITAXEL breast cancer HER-2 APOPTOSIS 维生素E琥珀酸酯 细胞凋亡 乳腺癌细胞 紫杉醇 HER 过表达 TUNEL法 VES
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