摘要
目的了解绵阳市食品中单增李斯特菌的污染及耐药状况,为预防控制该菌引起的食源性疾病提供科学依据。方法菌株分离采用GB 4789.30-2010方法,菌株鉴定采用API LISTERIA生化鉴定条,菌株药敏试验采用K-B法。结果 114份食品中共检出11株单增李斯特菌,总检出率为9.7%,生食水产品、生禽肉、生畜肉和豆制品4种食品受到该菌的污染,检出率分别为33.3%、26.7%、6.7%、6.7%。分离的11株菌株氨苄西林、阿莫西林/克拉维酸、复方新诺明、磺胺嘧啶、庆大霉素、阿米卡星、环丙沙星、头孢噻吩、氯霉素9种抗生素全部敏感;对头孢曲松和头孢噻吩没有敏感株。结论绵阳市的部分食品受到单增李斯特菌不同程度的污染,生食水产品受该菌污染最严重,其次是生禽肉,有发生食源性李斯特菌病的潜在风险。单增李斯特菌的耐药性较低,对多种抗生素敏感。应高度重视和加强食源性单增李斯特菌的污染状况及耐药性监测,以保证食品安全和人类健康。
[Objective]To know the contamination status and drug resistance of Listeria monocytogenes（LM） in food in Mianyang city,provide the scientific evidence for prevention and control of food-borne disease caused by Listeria monocytogenes. [Methods]The method of GB 4789.30-2010 was adopted to isolate LM,API-LISTERIA biochemical identification strips were applied to identify LM,and drug sensitivity test was conducted by K-B method. [Results]Among 144 food samples,11 strains of LM were detected,and the total detection rate was 9.7%.The raw aquatic products,raw poultry,raw meat and bean products were polluted by LM,which the detection rate was 33.3%,26.7%,6.7% and 6.7% respectively.All of 11 LM strains were sensitive to ampicillin,amoxicillin/clavulanic acid,compound sinomin,sulfapyrimidine,gentamicin,amikacin,ciprofloxacin,cephalothin and chloramghenicol.No strain was sensitive to cefatriaxone and cephalothin. [Conclusion]Some food was polluted by LM in Mianyang city,contamination status of raw aquatic products was the most serious,followed by raw poultry,and there was a potential risk of food-borne diseases of LM.The drug resistance of LM is low,and it is sensitive to several antibiotics.In order to ensure the food safety and human＇s health,it is necessary to pay attention to the contamination status of food-borne LM and strengthen the resistance surveillance.
出处
《职业与健康》
CAS
2011年第20期2330-2332,共3页
Occupation and Health
关键词
单增李斯特菌
污染状况
耐药性
食源性疾病
监测
Listeria monocytogenes
Contamination status
Drug resistance
Food-borne diseases
Surveillance
