摘要
研究野木瓜注射液(IS)及其提取物(AI)对氧化损伤脊髓神经元的保护和对未损伤脊髓神经元生长的影响.方法:通过MTT法和细胞外液LDH漏出量的测定检测IS对未损伤的脊髓神经元细胞存活率的影响;建立H2O2氧化损伤模型,通过MTT法、胞内SOD活力和细胞外液LDH漏出量的测定研究IS对H2O2氧化损伤脊髓神经元的细胞保护作用;运用激光共聚焦显微镜研究氧化受损及IS保护对脊髓神经元胞浆内Ca2+浓度变化的影响.结果表明:当浓度为100～200 mg/L时,IS能显著地促进离体培养的小鼠脊髓神经元氧化受损的修复,神经元存活率得到显著的提高,胞内SOD活力显著地增加和LDH漏出量显著地减少,同时胞内Ca2+超载显著地被抑制;未受损的脊髓神经元在200 mg/L IS作用下LDH漏出量显著地减少,细胞存活率显著地提高;AI3对能显著地提高氧化受损脊髓神经元的存活率.
Study the neuroprotective effects of Injection Stauntoniae （IS） and extracts on the oxidative damaged spinal neurons caused by H2O2 and its growth impetus on normal spinal neurons. The MIT assay, SOD activity and the leakage of LDH were used to observe the effects of IS on unmarred spinal neurons and the oxidative damage model used by H2O2. Besides, the changes of Ca^2＋ concentration in cytoplasm between damaged group and protected group was detected by LSCM. Within the concentration of 100 -200 mg/L,IS could increase the cell viability and SOD activity of oxidative damaged spinal neurons, and decrease the leakage of LDH in extracellular fluid as well as inhibit the Ca^2＋ concentration in cytoplasm of oxidative damaged spinal neurons in case of Ca^2＋ overloading. 200 mg/L IS could promote the growing of unmarred spinal neurons, decrease the leakage of LDH in extracellular fluid. AI3 could increase the cell viability significantly.
出处
《上海师范大学学报（自然科学版）》
2011年第5期540-545,共6页
Journal of Shanghai Normal University(Natural Sciences)
基金
上海师范大学科研项目(SK200715)
