摘要
目的:了解甘肃省脑炎脑膜炎症候群的病原种类及其分布特征。方法:对2009年6月-2011年3月甘肃省4市、州哨点医院送检的180例临床诊断为脑炎脑膜炎症候群患者的急性期脑脊液标本,用ELISA方法检测乙脑病毒IgM抗体,用多重PCR法检测乙脑病毒等7种病毒、脑膜炎奈瑟菌等5种细菌。结果:180例患者中的30例(16.67%)检测出相关病原,其中乙脑病毒IgM抗体阳性13例(7.22%),PCR检测,水痘-带状疱疹病毒7例(3.89%)人肠道病毒3例(1.67%)、EB病毒3例(1.67%)、C群脑膜炎奈瑟菌1例(0.56%)、巨细胞病毒1例(0.56%)、肺炎链球菌2例(1.12%),未检出人类疱疹病毒6型、单纯疱疹病毒1-2型、流感嗜血杆菌B型、B族链球菌、单增李斯特菌。结论:乙脑病毒是夏秋季甘肃省引起脑炎脑膜炎症候群的主要病原体,水痘-带状疱疹病毒次之,肠道病毒、EB病毒、肺炎链球菌、脑膜炎奈瑟菌也是重要的病原。
Objective:To use different diagnosis methods to study the pathogens and distributions of encephalitis and meningitis in Gansu province.Methods: 180 cerebrospinal fluid samples of the patients with the syndromes of encephalitis and meningitis were collected from four regions of Gansu province.Enzyme-linked immunosorbent assays(ELISA) was used to detect the IgM of Japanese encephalitis virus(JEV).Polymerase Chain Reaction(PCR) were used to detect the specific genes of JEV,Varicella-Zoster virus(VZV),Human enterovirus,Epstein-Barr virus(EBV),Cytoegalovirus(CMV),Human herpes virus 6(HHV6),Herpes simplex virus 1/2(HSV 1/2) and five bacteria(S.pneumoniae,H.influenzaetype b,N.meningitidis,Group B Streptococcus,L.monocytogenes).Results: 30 cases of 180 were positive(16.67%).IgM of JEV is 13 cases(7.22%).VZV is 7 cases(3.89%),Human enterovirus is 3 cases(1.67%),EBV is 3 cases(1.67%),CMV is 1 case(0.56%),S.pneumoniae is 2 cases(1.12%),N.meningitidis is 1 case(0.56%).Other pathogens were not found out.Conclusion: JEV was the main pathogen of encephalitis in Gansu,followed by VZV,Human enterovirus,EBV,S.pneumoniae and N.meningitidis.
出处
《中国卫生检验杂志》
CAS
2011年第10期2491-2492,共2页
Chinese Journal of Health Laboratory Technology
基金
国家科技重大专项传染病监测技术平台项目(2009ZX10004-208)
关键词
脑炎脑膜炎
脑脊液
病原
检测
Encephalitis and meningitis
Cerebrospinal fluid
Pathogens
Diagnosis