摘要
【目的】针对核桃育种中父本不清、亲本混淆问题,建立简便快捷高效的核桃亲本鉴定方法,也为其它林木和果树品种亲本鉴定提供参考。【方法】以‘鲁果2号’为鉴定材料,采用表型性状和SSR标记的方法对‘鲁果2号’及其可能的亲本进行亲子分析。【结果】表型性状方法统计的8对坚果性状在各品种间差异很小,不能鉴定出子代亲本;SSR分型检测出疑似亲本中只有‘上宋6号’和‘鲁香’与子代‘鲁果2号’的等位基因遗传符合孟德尔遗传规律,其累积RCP(亲子关系概率)为99.997%,‘slz-40’与已知亲本鉴定结果与实际相符。【结论】‘上宋6号’和‘鲁香’是‘鲁果2号’的亲本,SSR分型技术较好地鉴别出了‘鲁果2号’的亲本,为其它林木和果树品种寻找和鉴别亲本,提供有效手段。
【Objective】A simple,rapid and efficient walnut paternity method was established to resolve the problems of unknown paternal and confused parents in breeding,which provided reference for other varieties.【Method】＇Luguo-2＇ was used as material.Phenotypic traits and SSR markers were conducted to analyse parentage relationship between ＇Luguo-2＇ and its alleged parents.【Result】The phenotypes with statistics of eight nut stable expressional characters failed to get parent-child correlation results.In genotype results,the heredity of ＇Shangsong-6＇,＇Luxiang＇ and ＇Luguo-2＇ accords with Mendelian genetic regularity,there may be a paternity.PI and RCP wre analyzed by using the results of 10 SSR primer pairs,and the cumulative RCP was 99.997%.In addition,the appraisal result between ＇Slz-40＇ and its known parent was consistent with the reality.【Conclusion】＇Shangsong-6＇ and ＇Luxiang＇ were the parents of ＇Luguo-2＇ with SSR clustering results supporting the conclusion.The parents of ＇Luguo-2＇ were identified by SSR genotypes and thus providing an effective method to search and identify the parents of other trees and fruit varieties.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第20期4258-4264,共7页
Scientia Agricultura Sinica
基金
国家林业公益性行业科研专项经费项目:核桃集约及可持续生产关键技术研究(201004048)
国家林业局重点项目(2008-04)
