摘要
以鸭肝炎病毒(DHV)SN株VP1基因序列为基础,运用Garnier-Robson、Chou-Fasman和Karplus-Schulz方法预测VP1蛋白的二级结构,并分别运用Kyte-Doolittle方法、Jameson-Wolf方法和Emini方法预测蛋白的亲水性、抗原指数和表面可及性,最后结合吴玉章的方法综合分析预测其B细胞抗原表位。结果显示,VP1蛋白C端第132～137、177～186、209～219区段有较好的亲水性、表面可及性和较高的抗原指数,并且在二级结构上含有易形成抗原表位的无规则卷曲和β-转角,可能是VP1蛋白的B细胞抗原优势表位;DHV SN株与弱毒疫苗株在推测的VP1B细胞抗原表位177～186和209～219两个区域氨基酸序列变异较大。
The secondary structure of VP1 of duck hepatitis virus（DHV） SN strain was predicted with the methods of Garnier-Robson,Chou-Fasman and Karplus-Schulz.Its hydrophilicity,surface probability plot and antigenic index were obtained by the methods of Kyte-Doolittle,Emini and Jameson-Wolf,respectively.Then the method of WU Yuzhang was integrated to analyze the B cell epitopes.The results showed that the B cell epitopes of the VP1 protein were located at the C-terminal 132 to 137,177 to 186 and 209 to 219 regions according to the hydrophilicity plot,surface probability plot and antigenic index.There were obvious hereditary differences in VP1 C-terminal 177 to 186 and 209 to 219 regions between DHV SN strain and attenuated vaccine strain.
出处
《中国家禽》
北大核心
2011年第20期27-30,共4页
China Poultry
基金
国家自然科学基金(30170699)
国家自然科学基金(30871878)
