摘要
目的:利用斑蝥与红栓菌进行生物发酵转化的新方法,探索提高中药斑蝥中可利用的斑蝥素含量,增加斑蝥素的溶出率,从而扩大斑蝥资源的更有效利用。方法:以斑蝥素为标准品,用气相色谱法测定斑蝥中生物转化前后斑蝥素含量。采用HP-5气相色谱柱,香兰素为内标,柱温为175℃保持5 min,4℃/min升到240℃,保持5min,建立了斑蝥素含量测定方法。结果:建立的测定方法具有良好的重现性,转化前斑蝥中可溶出斑蝥素的含量为0.70%,转化后可溶出斑蝥素的含量为1.29%,转化前后测得斑蝥素的含量有明显提高。结论:利用红栓菌发酵对斑蝥素含量的提高具有重要的意义,为斑蝥资源的有效利用提供实验依据。
Objective：To compare the change of the content of cantharidin in Mylabris before and after biotransformation.Methods： The content of cantharidin was determined by gas chromatography（GC）.The conditions of GC were as follows： HP-5 column,vanillin as internal standard,the column temperature maintained at 175℃ for 5 min,rised to 240℃ at the speed of 4℃/min and the remained for 5 min.Results：The GC method was good reproducibility.The content of cantharidin in Myabris before and after botransformation was 0.7% and 1.29% respectively.After biotranformation,the content of cantharidin was increased.Conclusion： Mylabris fermentated with Trametes cinnabarina has great significance for increasing the content of cantharidn and provides experimentd basis for efficient use of Mylabris.
出处
《中药材》
CAS
CSCD
北大核心
2011年第8期1180-1182,共3页
Journal of Chinese Medicinal Materials
关键词
气相色谱法
斑蝥
斑蝥素
红栓菌
生物发酵与转化
含量
Gas chromatography
Mylabris
Cantharidin
Red Trametes
Fermentation and transformation
Content
