摘要
Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.
Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening （HCS） assays. Here we describe ethynyl deoxyuridine （EdU） as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.
基金
supported by the National Natural Science Foundation of China (30870535 and 90913017) (B. Zhang)
Introduced Innovative R&D Team Program of Guangdong Province (Gene Silencing Technology and Therapeutics)
