摘要
背景:肿瘤干细胞是否存在于所有肿瘤中仍有争议。目的:从人胃癌细胞中分离并鉴定类肿瘤干细胞,对胃癌的发生机制进行探讨。方法:原代培养胃癌细胞,并通过流式细胞术检测CD44和CD29等细胞因子的阳性表达率,分选阳性细胞进行成脂诱导。结果与结论:胃癌细胞中CD44、CD29的阳性百分率分别为5.67%和5.53%,CD44和CD29阳性细胞具有成脂能力,证明胃癌肿瘤细胞中存在具有分化能力的类肿瘤干细胞。
BACKGROUND：Whether cancer stem cells are present in all tumors is still controversial.OBJECTIVE：To isolate and identify cancer stem cells from human gastric cancer cells,and to explore the mechanism of gastric cancer.METHODS：Gastric cancer cells were primarily cultured and CD44 and CD29 positive expression rates were detected by flow cytometry to sort positive cells for adipogenic induction.RESULTS AND CONCLUSION：CD44 and CD29 positive percentages in gastric cancer cells were 5.67% and 5.53%,respectively,indicating that CD44 and CD29 positive cells had an adipogenic capacity.It is proved that there are tumor stem cells with differentiation capacity in gastric cancer cells.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第36期6718-6721,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
