摘要
在50 Hz 1.8 mT的4种不同波形电磁场(electromagnetic fields,EMFs)中筛选促进体外培养大鼠成骨细胞(rat osteoblasts,ROB)增殖与分化成熟的最佳波形.体外分离培养大鼠颅骨成骨细胞,传代后随机分为5组,分别用频率50 Hz,EMFs强度为0 mT(对照组)和1.8 mT的正弦波、三角波、方波和锯齿波处理ROB,30 min/(次.天).在磁场处理后4～8天细胞呈现特征样分布.方波促进成骨细胞增殖,正弦波抑制成骨细胞增殖.三角波和正弦波增加ALP活性,其中ALP染色、茜素红钙化结节染色和胶原Ⅰ(collagen-Ⅰ)免疫组织化学检测结果与ALP活性一致.在EMFs处理后的24 h、96 h和72 h后EMFs分别提高Runx-2、Opg和Igf基因表达水平,其中尤以正弦波和三角波作用最为显著.上述结果表明:50 Hz 1.8 mT方波促进成骨细胞增殖,正弦波抑制成骨细胞增殖.50 Hz 1.8 mT EMFs能促进体外培养成骨细胞分化成熟,其中尤以正弦波和三角波促进成骨细胞分化成熟作用最为显著.
To investigate the effect of 50 Hz 1.8 mT sinusoidal, triangular, serrate and square wave electromagnetic field （EMFs） on proliferation, differentiation and gene expression of osteoblasts in vitro, the newborn rat calvarial osteoblasts were isolated by enzyme digestion and randomly divided into 5 groups after one passage. The EMFs treatment group is exposed to 50 Hz 1.8 mT sinusoidal, triangular, serrate and square wave of EMFs and controls without EMFs treatment. The osteoblasts were exposed to the EMFs for 30 min/（time day）. The cells were observed under the contrast phase microscope each day. The EMFs groups were arranged in spiral appearance after 4- 8 days of the EMFs treatment. MTT result showed that sinusoidal wave significantly inhibited osteoblast proliferation, whereas square wave enhanced the osteoblast proliferation. The ALP activity in triangular and sinusoidal wave groups significantly increased （P 〈 0.01） compared with that in control. The number of calcified nodules, ALP stained result and Collagen- Ⅰ immunohistochemistry tested result are according to the result of ALP activity. There was a common result that sinusoidal and triangular wave significantly increased the Igf,, Opg and Runx-2 mRNA expression. The four different wave of EMFs at 50 Hz 1.8 mT enhances the osteoblasts maturation, mineralization and Opg and Runx-2 mRNA expression of the osteoblasts, while sinusoidal and triangular have the strongest effect.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2011年第10期967-974,共8页
Progress In Biochemistry and Biophysics
基金
甘肃省科技重大专项资助项目(09ZNKDA025)~~
关键词
成骨细胞
正弦波
三角波
锯齿波
方波
osteoblasts, sinusoidal wave, triangular wave, serrate wave, square wave
