摘要
目的:检测染色质重构复合物核心催化亚基(Brg1)及VEGF信号通路相关蛋白在Peutz-Jeghers综合征(PJS)中的表达,探讨其与PJS息肉发生发展的关系.方法:应用免疫组织化学染色技术分析72枚PJS息肉、12例正常小肠黏膜及30例小肠癌中Brg1、VEGF、COX-2蛋白的表达和分布,比较其在三种组织表达程度的差异.结果:Brg1、VEGF、COX-2在小肠腺癌组织中的阳性表达率明显高于PJS息肉组织和正常小肠黏膜组织(Brg1:76.67%vs54.17%,16.67%;VEGF:80.00%vs58.33%,8.33%;COX-2:83.33%vs62.50%,25.00%,均P<0.01).PJS息肉组织中Brg1与VEGF蛋白表达率呈显著正相关(χ2=15.734,r=0.467),与COX-2蛋白表达率呈正相关(χ2=7.552,r=0.324).结论:在PJS息肉中Brg1及VEGF信号通路可能参与了PJS的发生,并且是其癌变的重要因素之一.
AIM： To detect the expression of brahma-related gene 1 （Brg1）, vascular endothelial growth factor （VEGF） and cyclooxygenase 2 （COX-2） proteins in Peutz-Jeghers syndrome （PJS） and to analyze their clinical significance. METHODS： Immunohistochemistry was used to detect the expression of Brg1, VEGF and COX-2 proteins in 72 PJS samples, 12 normal small intestinal mucosal tissue samples and 30 cancer tissue samples. RESULTS： The positive rates of Brg1, VEGF and COX-2 expression were significantly higher in PJS than in normal mucosal tissue （54.17% vs 16.67%, 58.33% vs 8.33%, 62.50% vs 25.00%, all P 〈 0.01） and in cancer tissue than in PJS （76.67% vs 54.17%, 80.00% vs 58.33%, 83.33% vs 62.50% all P 〈 0.01）. In PJS, positive correlations were found between Brg1 and VEGF expression and between Brg1 and COX-2 expression. CONCLUSION： Brg1, VEGF and COX-2 may play a role in the occurrence of PJS.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第23期2461-2466,共6页
World Chinese Journal of Digestology
