摘要
目的探讨PRX-2转染对人成纤维细胞增殖特性的影响。方法取烧伤整形外科手术中所取的全厚皮片的剩余皮片，男5例，女3例。年龄6～41岁，平均24岁，均取自腹部。细胞培养采用组织块法，实验用第4代细胞。皮肤组织进行成纤维细胞的培养、传代，利用脂质体介导的基因转染技术，将PRX-2基因转染至人成纤维细胞中，形成稳定表达，利用MTT法、流式细胞仪等方法，观察转染前后人皮肤成纤维细胞增殖特性的变化情况。结果PRX-2转染后成纤维细胞形态与正常成纤维细胞类似，增殖能力明显增强，G0-G1期细胞比例减少，而S期和G2-M期细胞比例增多。PRX-2基因成功转染到了人成纤维细胞中；细胞增殖指数增高。结论脂质体介导的同源异形框基因PRX-2转染可以增强人成纤维细胞的增殖能力，PRX-2和人皮肤创面愈合有密切的关系。
Objective To explore the effects of PRX-2 gene transferred by lipofactamine on the human skin fihroblasts. Methods Normal human skin fibroblasts were cultured and PRX-2 gene was transferred by lipofactamine. The proliferation of fibroblasts was detected by MTT （3-（ 4,5-cimethylthiazol-2- yl）-2,5-diphenyl tetrazolium bromide） and flow cytometry. Results The proliferation of PRX-2-transfected fibroblasts was stronger than that of normal counterparts. There were fewer cells during G0-G1 period and more cells during S and G2-M periods. The proliferative index increased. Conclusion The proliferation of fibroblasts may be modified by transfected PRX-2. Thus PRX-2 plays an important role during the healing of human skin wound.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第37期2600-2603,共4页
National Medical Journal of China
基金
基金项目：国家自然科学基金（30000174）
中国博士后科研基金（2004035075）
