摘要
通过固体培养初选和摇瓶发酵复选,从腐烂的秸秆、腐草、腐树干中筛选获得酶活较高的真菌、放线菌和细菌各2株,将6个菌株分别接种到不同的碳源的牛羊粪中,经过2d培养后测定其CMC酶活。结果表明:不同菌种对同一粪便的酶活力不一样;同一菌株在不同粪便中表现的酶活力也不一样。其中,P2-6和P2-7在牛粪上酶活力最强,P2-6和B2-6及P2-7在羊粪上酶活力较强。最终确定以上3株菌株为牛羊粪堆肥发酵的首选菌株。
By screening in rotting straw,grass and trunk through solid culture preliminary and broth assay method,it obtained fungi, actinomycete and bacterial each two strain. Inoculation with each strain to cattle and sheep mature droppings, the CMC enzyme activity was measured after two days. The results showed that different strains behaved differently to the one mature;and it told the same trend for one strain to the different dung. It was found that CMC enzyme activity was strong on the condition of the fungi of P2-6 and P2-7 by using in two mature and bacterial of B2- 6 in sheep dung. Finally, the 3 strains were determined as the best ones for fermentation cattle and sheep mature.
出处
《北方园艺》
CAS
北大核心
2011年第20期58-60,共3页
Northern Horticulture
关键词
牛羊粪
纤维素分解菌
酶活
筛选
cattle and sheep mature
cellulose-decomposing microorganisms
enzyme activity
screening
