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RNAi技术阻抑CTGF对瘢痕疙瘩形成影响的体外研究 被引量：1

Research on the effects of RNAi in inhibiting CTGF on the formation of keloid in vitro
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摘要 目的 用RNA干扰（RNAi）技术探讨结缔组织生长因子（CTGF）对瘢痕疙瘩中胶原代谢的影响.方法 采用RNAi技术,将针对人CTGF基因设计合成的3对特异性小干扰RNA（siRNA）-CTGF分别转染体外培养的人瘢痕疙瘩成纤维细胞（KFB）,通过逆转录-多聚酶链反应（RT-PCR）和Western blot检测转染后瘢痕疙瘩组织中CTGF表达量的变化及胶原含量的变化,并与对照组比较.结果 第3对（C3）siRNA-CTGF转染人KFB后CTGF的基因表达及胶原蛋白表达水平显著受抑,其抑制率分别为86.8%及65.6%.结论 质粒siRNA-CTGF转染人KFB后有效沉默CTGF的表达进而使胶原合成降低,CTGF对瘢痕疙瘩形成过程中胶原蛋白的合成有促进作用.
作者 李雪阳 金培生 沈才齐 张爱君 陶常波
机构地区 徐州医学院附属医院整形外科
出处 《徐州医学院学报》 CAS 2011年第8期511-514,共4页 Acta Academiae Medicinae Xuzhou
基金 江苏省卫生厅课题（Z200705）
关键词 瘢痕疙瘩成纤维细胞 RNA干扰 结缔组织生长因子
分类号 R622.9 [医药卫生—整形外科]
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参考文献11

  • 1Alster TS,West TB. Treatment of scars: a review [ J ]. Ann Plast Surg,1997,39(4) :418 -432.
  • 2Shetlar MR, Shetlar DJ, Bloom RF, et al. Involution of keloid im- plants in athymic mice treated with pirfenidone or with triamcinolo- ne [J]. JLabClinMeb,1998,132(6):491-496.
  • 3Linares HA. From wound to scar [J]. Bums, 1996,22 (5) :339 - 352.
  • 4李世荣,刘剑毅,纪淑兴.体外培养人增生性瘢痕成纤维细胞胶原合成及结缔组织生长因子的表达[J].中华整形外科杂志,2004,20(2):124-127. 被引量：11
  • 5Bradham DM, Igarashi A, Potter RL, et al. Connective tissue growth factor: a cysteine - rich mitogen secreted by human vascu- lar endothelial ceils is related to the SRC - induced immediate early gene product CEF - 10 [ J ]. J Cell Biol, 1991,114 ( 6 ) : 1285 - 1294.
  • 6Grotendorst GR, Okochi H, Hayashi N. A novel transforming growth factor 13 response element controls the expression of the connective tissue growth factor gene [J] Cell Growth Diffe, 1996,7(4) :469 -480.
  • 7陶常波,金培生,张爱君,李雪阳.结缔组织生长因子在病理性瘢痕中的表达[J].徐州医学院学报,2009,29(12):828-830. 被引量：8
  • 8Pearson DS, Kulyk WM, Kelly GM, et al. Cloning and character- ization of a cDNA encoding the collagen - binding stress protein hsp47 in zebrafish [ J ]. DNA Cell Biol, 1996,15 (3) :263 - 272.
  • 9Hammond SM, Bemstein E, Beach D, et al. An RNA - directed nuclease mediates post - transcriptional gene silencing in Drosophi- la cells [ J ]. Nature,2000,404 (6775) :293 - 296.
  • 10Zamore PD, Tuschl T, Sharp PA, et al. RNAi: double - stran- ded RNA directs the ATP - dependent cleavage of mRNA at 21 to 23 nueleotide intervals [ J ]. Ceil ,2000,101 ( 1 ) :25 - 33.

二级参考文献20

  • 1Letterio JJ, Bottinger EP. TGF - β knockout and dominant - negative receptor transgenic mice [ J]. Miner Electrolyte Metab. 1998, 24(2 -3) :161 -167.
  • 2Chiou S, Yoo J, Loh KC, et al. Identification of rat mammary tumor - 1 gene ( RMT - 1 ) , which is highly expressed in rat mammary tumors [J]. Cancer Dett, 2001, 174(3) :45 -55.
  • 3Border WA, Noble NA. Transforming growth factor beta in tissue fibrosis [J]. N Engl J Med, 1994, 331 (19) : 1286 -1292.
  • 4Fan WH, Kamovsky MJ. Increased MMP -2 expression in connective tissue growth factor over - expression vascular smooth muscle cells [J]. J Biol Chen, 2002, 277 (12) :9800 -9805.
  • 5Bradham DM, Igarashi A, Potter RL. Connective tissue growth factor: a cystine -rich mitogen secreted by human vascular endothelial ceils is related to the SRC -induced immediate early gene product CEF- 10 [J]. J Cell Biol, 1991, 114 (6):1285- 1294.
  • 6Chaqour B, Goppelt - Struebe M. Mechanical regulation of the Cyr 61/CCN1 and CTGF/CCN2 proteins [J]. FEBS J, 2006, 273 (16) : 3639 - 3649.
  • 7Wang JF, Olson ME, Ball DK, et al. Recombinant connective tissue growth factor modulates porcine skin fibroblast gene expression [J]. Wound Repair Regen, 2003, 11 (3) :220 - 229.
  • 8Shimo T, Nakaishi T, Nishida T, et al. Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial ceils in vitro, and angiogenesis in vivo [ J]. J Biochem, 1999, 126( 1 ) :137 - 145.
  • 9Rodriguez - Vita J, Ruiz - Ortega M, Ruperez M, et al. Endothelin - 1, via ETA receptor and independently of transforming growth factor - β, increases the connective tissue growth factor in vascular smooth muscle cells [J]. Cite Res, 2005, 97(2) :125 -134.
  • 10Blom IE, Goldschmeding R, Leask A. Gene regulation of connective tissue growth fctor: new targets for antifibrotic therepy? [ J]. Matrix Biol, 2002, 21 (6) :473 -482.

共引文献16

同被引文献22

  • 1刘剑毅,李世荣,纪淑兴.CTGF反义寡核苷酸对人瘢痕疙瘩成纤维细胞的作用[J].中华整形外科杂志,2004,20(6):454-456. 被引量：15
  • 2Leask A,Abraham DJ. The role of connective tissue growth factor,a multifunctional matricellularprotein,in fibroblast biology [J].Biochem Cell Biol, 2003,81 (6) : 355-363.
  • 3Brandham DM,Igarashi A, Potter RL.Connective tissue growth factor: acysteine-rich mitogen secreted by human vascular endothelial cell is related to the SRC-induced immediate early gene product CEF-10[J]. Cell Biol, 1991,14(6) : 1285-1294.
  • 4Grotendorst GR, Okochi H, Hayashi N.A novel transforming ga'owth factor 13 response element controls the expression of the connective tissue growth factor gene[J].Cell Growth Differ, 1996,7 (4) :469.
  • 5Shi WX,Pennington D,et al.Autocrine overexpression of CTGF maintains fibros is:RDA analysis of fibrosis genes in systemic sclerosis [J]. Experimental Cell Research, 2000,259 ( 1 ) :213-214.
  • 6Wayne KS, Alexander GD, Gordon RT,et al. Physiology and healing dynamicsofchroniccutaneouswounds [J].AmJSurg, 1998,176 (Suppl2 A)26-3811 Physiol, 1996,167(3 ) : 500-511.
  • 7王关蒿,钱桂生,关松,等.JAK-STATs信号传导及临床意义[J].中国老年学杂志,2003,22(1):70-72.
  • 8But'ridge K, Wennerberg K. Rho and Rac take center stage [J]. Cell, 2004,116(2): 167-179.
  • 9Tanaka T, Nishimura D, Wu RC, et al. Nuclear Rho kinase, ROCK2, targets P300 acetyltransferase [J].J Biol Chem.2006,281 (22): 15320-15329.
  • 10Wang H, Eto M, Steers WD, et al. RhoA-mediated Ca2 sensitization in erectile function[J].J Biol Chem, 2002,277 (34) : 30614-30621.

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徐州医学院学报
2011年 第8期

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