摘要
采用聚乙二醇(PEG 4000)/硫酸盐双水相体系,经一次萃取从地木耳细胞破碎液中富集分离藻蓝蛋白.分别考察萃取时间、硫酸盐种类及浓度、PEG 4000浓度、溶液pH值和离子强度对双水相萃取分离地木耳中藻蓝蛋白的影响.结果表明:在萃取时间为30min,Na2SO4的质量分数为15%,PEG 4000的质量分数为12%,加入KCl的质量分数为1%,pH值为3.0的最优条件下,藻蓝蛋白萃取率可达95.2%,纯度为4.8.
Enrichment and separation of phycocyanin from nostoc cell homogenates was studied under polyethylene glycol （PEG 4000）/sulphate aqueous two-phase system by one step extraction. The effects of time, type and concentration of sulphate, the concentration of PEG 4000, ionic strength and pH value on the extraction behavior were investigated respectively. The optimized extraction rate of phycocyanin from nostoc was 95.2% and reached the purity of 4.8, and the optimum conditions for extraction were extraction time of 30 min, Na2SO4 mass fraction of 15%, PEG 4000 mass fraction of 12%, KC1 mass fraction of 1% with pH value of 3. 0.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2011年第6期672-675,共4页
Journal of Huaqiao University(Natural Science)
基金
华侨大学科研基金资助项目(08X0211*)
关键词
地木耳
藻蓝蛋白
聚乙二醇
双水相体系
萃取
nostoc
phycocyanin
polyethylene glycol
aqueous two-phase system
extraction
