摘要
分别以争议小牛和嫌疑母牛的基因组总DNA为模板,运用STR复合扩增技术对TGLA227、BM2113、TGLA53、ETH10、SPS115、TGLA126、TGLA122、INRA23、ETH3、ETH225、BM1824共11个STR(短串联重复序列)基因座进行扩增,扩增产物经遗传分析仪检测,检测结果通过GeneMapper片段分析软件分析,排除了嫌疑母牛与争议小牛间具有亲子关系的可能性。
Taking the genomic DNA of disputable calf and suspicion cow as template respectively,the amplifications of eleven STR（short tandem repeat） loci（TGLA227,BM2113,TGLA53,ETH10,SPS115,TGLA126,TGLA122,INRA23,ETH3,ETH225 and BM1824） were carried out by using the multiplex amplification technique of STR,and the PCR products were further separated and analyzed in a genetic analyzer,then the detection results were analyzed by GeneMapper fragment analysis software.The result was that the probability of parentage between the disputable calf and suspicion cow was ruled out.
出处
《江西农业学报》
CAS
2011年第10期23-24,共2页
Acta Agriculturae Jiangxi
基金
科技部林业公益项目(201004094)
南京森林警察学院科研基金项目(QN201109)
关键词
STR分型技术
亲子鉴定
牛
STR loci analysis
Parentage identification
Cattle
