摘要
目的:探讨灵芝孢子油对D-半乳糖所致衰老模型小鼠抗衰老作用及其作用机理。方法:以D-半乳糖建立小鼠衰老模型,灌胃灵芝孢子油900mg/(kg.d)45d后,检测小鼠血清中血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性;检测脾脏指数、胸腺指数;半定量PCR检测脾脏、胸腺中C3、P21 mRNA表达。结果:DB组(D-半乳糖+灵芝孢子油)SOD、CAT的活性显著高于DY组(D-半乳糖+玉米油)(P≤0.01),DB组脾脏指数、胸腺指数显著高于DY组(P≤0.05)。C3mRNA在脾脏、胸腺中的表达DB组显著高于DY组(P≤0.05),C3mRNA在胸腺中的表达SB组(生理盐水+灵芝孢子油)高于SY组(生理盐水+玉米油)(P≤0.05);P21mRNA在脾脏中的表达DB组低于DY组(P≤0.05),SB组低于SY组(P≤0.05)。结论:灵芝孢子油可提高小鼠脾脏指数、胸腺指数、SO D、C AT活性,具有抗氧化功能;能促进C3 mR NA的表达,抑制P 21 mR NA的表达。
Objective： To explore the immunoregulation effect of Ganoderma lucidum spore oil in aged mouse models induced by D-galactose and its anti-aging mechanisms.Methods： Aged mice were intragastrically administered with Ganoderma lucidum spore oil for 45 consecutive days to examine the change of SOD activity,CAT activity,spleen index and thoracic gland index.Meanwhile,the mRNA expression levels of C3 and P21 in mouse spleen and thymus were determined by semi-quantitative RT-PCR.Results： The SOD activity and CAT activity in DB（D-galactose ＋ Ganoderma lucidum spore oil） group were much higher than those in DY（D-galactose ＋ corn oil） group（P≤0.05）.The spleen index and thoracic gland index in DB group were higher than those in DY group（P≤0.01）.The mRNA expression levels of C3 in spleen and thymus was significantly higher in DB group than those in DY group（P≤0.05）;similarly,the thymus mRNA expression levels of C3 in SB（normal saline ＋ Ganoderma lucidum spore oil） group was significantly higher than that in SY（normal saline ＋ corn oil） group（P≤0.05）.Spleen P21 mRNA expression level was significantly lower in DB group compared with DY group（P≤0.05） and in SB group compared with SY group（P≤0.05）.Conclusion： Ganoderma lucidum spore oil can improve spleen index and thoracic gland index,SOD activity and CAT activity.It also can stimulate the expression of C3 and inhibit the expression of P21,which may be one of the antioxidant mechanisms of Ganoderma lucidum spore oil.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第19期232-235,共4页
Food Science
基金
湖南省人事厅湖南省博士后基金项目(0971111)
关键词
灵芝孢子油
衰老
免疫
小鼠
Ganoderma lucidum spore oil
aging
immunity
mouse
