摘要
为促进芭蕉芋良种的推广使用及芭蕉芋产业的健康发展,以芭蕉芋为材料进行了组培技术及块茎芽片繁殖技术的研究。结果表明,芭蕉芋茎尖诱导的培养基最佳组合为MS+6-BA 2.5mg/L+NAA0.5mg/L+琼脂8g/L+蔗糖30g/L+GA30.5mg/L+ZT 2.0mg/L+DVP 0.5g/L(或AC 0.5g/L),其出苗率为47.4%;生根培养基最佳组合为1/2MS+6-BA 2.0mg/L+NAA 0.5mg/L+琼脂8g/L+蔗糖30g/L+GA30.5mg/L+ZT 2.0mg/L+DVP 0.5g/L,其苗的成活率达86.0%;增殖培养基最佳组合为MS+6-BA 2.0mg/L+NAA 0.5mg/L+琼脂8g/L+蔗糖30g/L+GA30.5mg/L+ZT 2.0mg/L+DVP0.5g/L,其繁殖倍数为14.98。芭蕉芋块茎芽片苗经NAA 0.25mg/L浸泡30min的发芽率最高,为65.0%;芭蕉芋顶部芽的出苗率优于底部芽,顶部芽的芽片苗的生长情况优于底部芽。
The technique of tissue culture and rapid propagation technique in C.edulis was studied to promote popularization of improved C.edulis varieties and healthy development of C.edulis industry.The results showed that the optimum media for stem apex induction,rooting and propagation were MS＋2.5 mg/L 6-BA＋0.5 mg/L NAA＋8 g/L agar＋30 g/L sucrose＋0.5 mg/L GA3＋2.0 mg/L ZT＋0.5 g/L DVP（or AC 0.5 g/L）,1/2 MS＋2.0 mg/L 6-BA＋0.5 mg/L NAA＋8 g/L agar＋30 g/L sucrose ＋0.5 mg/L GA3＋2.0 mg/L ZT＋0.5 g/L DVP and MS＋2.0 mg/L 6-BA＋0.5 mg/L NAA＋8 g/L agar＋30 g/Lsucrose＋0.5 mg/L GA3＋2.0 mg/L ZT＋0.5 g/L DVP separately.And the induction rate,plantlet survival rate and propagation time were 47.4%,86.0% and 14.98 respectively,the germination rate of buds soaked in 0.25 mg/L NAA for 30 min could reach 65.0%,the plantlet rate and growth vigor of plantlets cultured from the top buds were better than those of plantlets cultured from the bottom buds.
出处
《贵州农业科学》
CAS
北大核心
2011年第10期47-50,共4页
Guizhou Agricultural Sciences
基金
贵州省科技厅项目"贵州生物质能源植物研发创新能力建设"[黔科合院所创能(2010)4007]
贵州省农业科学院专项"芭蕉芋良种块茎快繁技术研究与应用"[院ZX(2007)027]
关键词
芭蕉芋
块茎
组培
快繁技术
Cannas edulis
tuber
tissue culture
rapid propagation
