摘要
目的:建立同时测定紫果西番莲叶中荭草素、异荭草素与牡荆素含量的方法。方法:采用高效液相色谱法。色谱柱为Inertsil C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(梯度洗脱),检测波长为350nm,流速为1mL·min-1,柱温为30℃。结果:异荭草素、荭草素与牡荆素的检测浓度分别在0.015～0.150mg·mL-1(r=0.9998)、0.012～0.120mg·mL-1(r=0.9998)、0.012～0.120mg·mL-1(r=0.9994)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为99.04%(RSD=0.80%,n=6)、99.30%(RSD=1.03%,n=6)、98.92%(RSD=1.11%,n=6)。结论:本方法简便、准确,可为紫果西番莲叶的进一步开发利用提供质量控制依据。
OBJECTIVE： To develop a method for the simultaneous determination of orientin, isoorientin and vitexin in the leaves of Passiflora edulis. METHODS： HPLC method was adopted. Intertsil C18（250 mm×4.6 mm, 5 μm） colunm was used with mobile phase consisted of acetonitrile-0.1% phosphoric acid （gradient elution） at the flow rate of 1 mL. min〈 respectively. The detection wavelength was set at 350 nm and the column temperature was 30 ℃. RESULTS： The linear ranges of isoorientin, orientin and vitexin were 0.015-0.150 mg.mL 1（r=0.999 8）, 0.012-0.120 mg.mL^-1（r=0.999 8） ,0.012-0.120 mg.mL^-1（r=0.999 4）, respectively. The average recoveries were 99.04% （RSD=0.80%, n= 6）, 99.30% （RSD= 1.03 %, n-= 6）, 98.92% （RSD= 1.11%, n= 6）. CONCLUSION： The method is simple, accurate and available for quality control basis of the further development of the leaves ofP. edulis.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第43期4075-4076,共2页
China Pharmacy
基金
广东省中医药局2010年建设中医药强省立项资助课题(2010253)
