摘要
目的:研究滇重楼中4种重楼皂苷的含量与其生长年限的关系。方法:采用高效液相色谱法测定不同生长年限滇重楼中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的含量。色谱柱为Agilent Zorbax SB-C18(150mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1.0mL·min-1,检测波长为203nm,柱温为35℃。结果:不同生长年限滇重楼中重楼皂苷的含量差异较明显,3年生药材重楼皂苷的含量比较低,4、5年生药材含量明显升高,5年以上药材含量有下降趋势。结论:重楼皂苷含量的变化规律可为确定滇重楼采收年限提供参考依据。
OBJECTIVE: To investigate the relationship of 4 kinds of chonglou saponins contents in Paris polyphylla with different growing years. METHODS: HPLC method was adopted to determine the contents of chonglou saponins Ⅰ、Ⅱ、Ⅵ、Ⅶ in P. polyphylla of different growing years. Agilent Zorbax SB-C18(150 mm×4.6 mm, 5 μm) column was used with mobile phase composed of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL. min 1. The detection wavelength was set at 203 nm, and the column temperature was 35 ℃. RESULTS: The diversity of the content of chonglou saponins in P polyphylla of different growing years was great. The ingredients in 3 years of P. polyphylla were in the lower levels, the ingredients in 4 and 5 years increased significantly, and the ingredients in over 5 years had a decreasing tendency. CONCLUSION: The changing rules of 4 chonglou saponins in P. polyphylla provide scientific basis for the collecting year.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第43期4081-4083,共3页
China Pharmacy
基金
云南省教育厅科学研究基金一般项目(09Y0361)
大理市科技局科技项目(2009SF08)
大理学院青年教师科研基金项目(KY-QN2009-39)