摘要
本文本以球型松紧不同基因型花椰菜自交系为材料,研究了不同浓度NaCl(0、68、136 mmol.L-1)胁迫对花椰菜幼苗生长、水分生理、光合色素以及质膜透性的影响。结果表明:盐胁迫,尤其是高浓度NaCl(136 mmol.L-1)胁迫,显著降低了花椰菜幼苗的株高生长量、叶片长宽、植株干鲜重量、植株相对含水量(PRWC)、叶片相对含水量(LRWC)、细胞膜稳定指数(CMSI)、叶绿素(Chl b)、总叶绿素(Chl)、类胡萝卜素(Car)含量和类胡萝卜素/总叶绿素(Car/Chl);显著提高了叶片丙二醛(MDA)含量和叶绿素a/b(Chl a/Chl b);叶绿素a(Chl a)含量变化因品种和盐浓度而异。盐胁迫抑制了花椰菜幼苗的水分吸收和光合色素合成,同时破坏了叶片细胞膜的完整性,进而影响了其幼苗的生长发育。与对照(0 mmol.L-1 NaCl)相比,不同浓度NaCl胁迫对幼苗的株高生长量、干鲜重量、PRWC、LRWC、CMSI、Chl b和Chl含量的降幅及MDA含量的增幅均为YM-80<RZ-50,表明两自交系的耐盐性YM-80>RZ-50。
In this paper,using the different genotypes of cauliflower seedlings（RZ-50 and YM-80） as tested materials,plant growth,water physiology,photosynthetic pigment and membrane permeability were investigated under different concentrations of NaCl（0,68 and 136 mmol·L-1）.The result showed that the seedling height increment,leaf length and width,seedling fresh weight and dry weight, relative water content of plant and leaf,cell membrane stability index（CMSI）,contents of chlorophyll b（Chl b）,chlorophyll and carotenoid and Car/Chl ratio were reduced obviously by salt stress,especially at high concentration of NaCl（136 mmol·L-1）.However,the content of malondialdehyde（MDA） and chlorophyll a/b（Chl a/Chl b） ratio increased significantly.While content of chlorophyll a（Chl a） changed differently with NaCl concentration or the cultivars. These results indicated that the decline of seedling growth was caused mainly by inhibition of water absorption,photosynthetic pigment synthesis and membrane integrity under salt stress. Moreover,a higher degree of the changes for seedling height increment,seedling weight,PRWC, LRWC,CMSI,Chl b,Chl and MDA was caused by NaCl in RZ-50 compared with that in YM-80.Therefore, salinity stress had stronger influence to RZ-50.The salt tolerance of YM-80 would be higher than that of RZ-50.
出处
《西南农业学报》
CSCD
北大核心
2011年第5期1722-1727,共6页
Southwest China Journal of Agricultural Sciences
基金
浙江省教育厅科研项目(Y200906705)
浙江省科技计划项目(2008C22094)
关键词
花椰菜
盐胁迫
叶绿素
MDA
幼苗生长
细胞膜稳定指数
Cauliflower
Salt stress
Chlorophyll
MDA
Seedling growth
Cell membrane stability index
