摘要
应用SRAP分子标记技术对87份来茄子及其近缘野生种进行了遗传多样性分析。结果显示,从88对SRAP引物中筛选出18对多态性高、稳定性好的引物组合,共检测出309清晰个位点,平均每对引物检测到17.2个扩增位点。.参试茄属植物种质群体位点的平均杂合度为0.6962;平均多态信息含量为0.6441。82份栽培种茄子材料的平均相似系数为0.822,表明茄子栽培种的基因库较小,遗传基础较为狭窄。聚类分析结果表明,87份材料可分成4大类,可以较好地将茄子栽培种与其近缘野生种分开,并且基本可将高级栽培种(S.melongena L.subsp.melongena)和原始栽培种(S.melongena L.subsp.ovigerum Salis)在亚种水平上区分开来。由此可见,SRAP标记在茄子遗传研究中是一种经济、有效和可靠的分子标记手段。
The genetic diversity of 87 accessions including 82 Solanum melongena L.（inluding S.melongena L.subsp.melongena L.and S.melongena L.subsp.ovigerum Salis） and relate species （S.integrifolium L.and S.macaonense L.） were revealed by SRAP markers.18 of the 88 primers were employed and showed polymorphism and stability.Total 309 alleles were screened,the average number of alleles per primer were 17.2.The heterozygosity average value,and average of polymorphism information content of 309 SRAP loci were 0.6962,and 0.6441.Phenetic tree were constructed using Jaccard＇s coefficient and UPGMA and the analysis of cluster suggested that 87 accessions could be divided in to four groups.Average similarity of 82 cultivar eggpant was 0.822, which indicated their genetic relationship was close.The subgrops of cultivar specie（S.melongena L.） were almost differentiated by the SRAP marker.All these results showed that SRAP markers were economic,effective,and reliable.
出处
《西南农业学报》
CSCD
北大核心
2011年第5期1853-1860,共8页
Southwest China Journal of Agricultural Sciences
基金
国家大宗蔬菜产业技术体系专项资金项目(CARS-25)
四川省"十二五"农作物育种攻关项目
四川省"十二五"基因工程财政专项项目
