摘要
目的总结无关供者外周血干细胞(PBSC)动员和采集情况。方法 24例无关供者给予重组人粒细胞集落刺激因子(rhG-CSF)5μg.kg-1.d-1,每天皮下注射,第4、5天或5、6天用CS-3000PLUS血细胞分离机采集外周血干细胞,计数采集物中单个核细胞(MNC)和CD34+细胞。结果所有供者均安全顺利完成了PBSC动员和采集,收获的MNC为(5.57±1.19)×108/kg,CD34+细胞为(3.81±1.25)×106/kg受者,患者10～20 d获得造血重建。结论 5μg.kg-1.d-1的rhG-CSF动员PBSC安全有效。
Objective To summarize the result of peripheral blood stem cell（PBSC） mobilization and col- lection in 24 unrelated donors. Methods Subcutaneous injection of recombinant human granulocyte colony-stimulating factor（rhG-CSF）was given to 24 unrelated donors for 4 - 5 or 5 - 6 days and CS-3000PLUS model blood cell separator was used to collect PBSC. Mononuclear cell （MNC） and CD34 ＋ cell count from the sample of harvest were measured. Results PBSC mobilization and collection were successfully completed in 24 unrelated donors, The harvested MNC and CD34 ＋ cell count were（5.57±1.19）×10^8/kg and（3.81±1.25）×10^6/kg recipient separately. The recipient patients hematological reconstitution time occurred from 10 to 20 days after PBSC transplantation. Conclusion PBSC mobilization using 5μg·kg^-1·d^-1 rhG-CSF is safe and effective.
出处
《中国临床保健杂志》
CAS
2011年第5期484-486,共3页
Chinese Journal of Clinical Healthcare
关键词
外周血干细胞移植
供血者
造血干细胞动员
血样采集
Peripheral blood stem cell transplantation
Blood donors
Hematopoietic stem cell mobilization
Blood specimen collection
