摘要
目的:从脑-肠轴角度探讨针刺在预防和治疗应激性胃黏膜损伤方面的作用机制。方法:将雄性Wistar大鼠随机分为正常组、模型1组、模型2组、治疗组、预防组,每组8只。采用乙醇灌胃法造成大鼠应激性胃黏膜损伤模型。治疗组先造模后针刺,预防组先针刺后造模;模型1组和模型2组的造模时间分别同治疗组和预防组。穴选"足三里""中脘""内关",针刺干预5 d。实验结束前1 h各组用10%炭粉混悬液灌胃,然后取材,计算炭粉推进百分率(胃肠推进百分率),肉眼观察大鼠的胃黏膜形态,Guth法计算胃黏膜损伤指数,酶联免疫法测定血浆和下丘脑中β-内啡肽的含量。结果:与正常组比较,两个模型组的胃肠推进百分率均明显降低(P<0.05),肉眼可见胃黏膜损伤明显,损伤指数明显升高(P<0.05,P<0.01),血浆及下丘脑中β-内啡肽含量均明显升高(P<0.01);与相应的模型组相比,预防组的胃肠推进百分率显著提高(P<0.05),治疗组和预防组大鼠的胃黏膜损伤程度明显减轻,损伤指数明显降低(P<0.05,P<0.01),血浆中β-内啡肽含量明显降低(P<0.05,P<0.01),下丘脑中β-内啡肽含量显著升高(P<0.01)。结论:针刺能促进胃黏膜损伤的修复,提高胃肠功能,与其降低血浆中β-内啡肽含量,增加下丘脑中β-内啡肽含量有关。针刺"足三里""中脘""内关"预防胃黏膜损伤的效果明显。
Objective To explore the mechanism of acupuncture in preventing and treating stress-induced gastric mucosal injury from the view of brain-gut axis.Methods Forty male Wistar rats were randomly divided into normal control,model 1,treatment,model 2 and prevention groups（n=8）.Gastric mucosa injury model was established by intragastric perfusion of dehydrated alcohol（1.0 mL/rat）.Rats of the treatment group were treated with acupuncture after modeling,while those of the prevention group treated first,followed by modeling.The time of modeling in model 1 group and model 2 group was simultaneous with that of the treatment group and prevention group respectively.Acupuncture was applied to ＂Zusanli＂（ST 36）,＂Zhongwan＂（CV 12） and ＂Neiguan＂（PC 6） for 20 min,once daily for 5 days.Before sampling the tissues,10% charcoal suspension was intragastric perfused 1 h for analyzing the rate of gastrointestinal propulsion（distance from the upper end of the charcoal powder to the cardia / total length of the cardia to the anus×100%）.Gastric mucosal ulcer index was measured by using Guth＇s method.The contents of β-endorphin（β-EP）in plasma and hypothalamus were determined by enzyme-linked immunosorbent assay.Results Compared with the normal control group,the gastrointestinal propulsion rates were decreased considerably in the two model groups（P0.05）,while the gastric mucosal ulcer indexes and the contents of β-EP in both plasma and hypothalamus were increased significantly in the model groups（P0.05,P0.01）.Compared with the corresponding model groups,the gastrointestinal propulsion rate was increased remarkably in the prevention group（P0.05）,and the gastric mucosal ulcer indexes and the contents of plasma β-EP level were decreased obviously in both treatment and prevention groups（P0.05,P0.01）.The contents of hypothalamic β-EP were increased further in the later two groups（P0.01）.Conclusion Acupuncture of ST 36,CV 12 and PC 6 can promote the repair of gastric mucosal injury and improve gastrointestinal function,which may be related to its effects in reducing plasma β-EP and upregulating hypothalamic β-EP level.Acupuncture also has a better effect in preventing gastric mucosal injury.
出处
《针刺研究》
CAS
CSCD
北大核心
2011年第5期341-346,共6页
Acupuncture Research
关键词
应激性胃黏膜损伤
针刺
胃肠推进率
胃黏膜损伤指数
血浆及下丘脑β-内啡肽
Stress-induced gastric mucosal injury
Acupuncture
Gastrointestinal propulsion rate
Gastric mucosal ulcer index
Plasma and hypothalamus β-endorphin
