摘要
目的探讨碱性成纤维细胞生长因子(bFGF)基因修饰的组织工程化复合物对牙龈组织缺损再生的影响。方法本研究于2007年7月至2009年1月在福建省高校口腔医学重点实验室进行。利用bFGF基因转染Beagle犬牙龈成纤维细胞(GFs),并将其接种于脱细胞真皮基质(ADM)形成组织工程化复合物,植入Beagle犬的人工牙周组织缺损区,通过对治疗前后牙周指标测量分析,评价该组织工程化复合物对牙龈组织再生的影响。结果实验前后附着丧失差值测定显示GFs与ADM复合物组及转染bFGF基因的GFs复合物组之间无显著性差异,与空白对照组相比均有明显增加(P<0.05)。角化龈宽度差值,6周时转染基因组有明显增加,12周时两实验组均比空白对照组明显增加(P<0.05)。结论复合GFs的ADM可能有助于改善附着丧失的程度及角化龈宽度,而bFGF的作用并不肯定。组织工程技术能有效促进牙龈组织再生。
Objective To study the effects of a tissue-engineered complex modified by basic fibroblast growth factor（bFGF） gene on gum regeneration. Methods The study was carried out in Fujian Provincial Key Laboratory of Stomatology from July 2007 to January 2009. The gingival fibroblasts （GFs）of Beagles were transfected with bFGF gene and incubated on the acellular dermal matrix （ADM）to form a tissue-engineered complex. The complex was placed into the periodontal defect and the effects of tissue regeneration were evaluated by histological examination. Results The attachment loss examination showed that there was no significant difference between gene-modified GFs-ADM complex group and gene-modified GFs group, but the attachment loss in blank control group were significantly higher than the other two groups （P 〈 0.05）. At week 6, the width of keratinized gingival margin in the gene-modified GFs group increased significantly and later at week 12, the width in the two experimental groups significantly increased compared with the control group （P 〈 0.05）. Conclusion The GFs-ADM composite may help to reduce the attachment loss and decrease keratinized gingiva width. However,the effect of bFGF is not certain. The tissue engineering technology can promote gum regeneration.
出处
《中国实用口腔科杂志》
CAS
2011年第10期602-605,共4页
Chinese Journal of Practical Stomatology
基金
国家自然科学基金(30471892)
福建省科技厅
福建医科大学科学研究发展基金(XZ04011)
