摘要
目的应用藏茵陈对MHV-A59病毒感染湿热证小鼠模型进行治疗,通过实验初步探讨该药对小鼠病毒性肝炎的干预机制。方法将32只小鼠随机分为正常组、模型组、阳性组和藏茵陈组。分别在造模前后测定小鼠的肛温和体重,并在造模后分别用试剂盒测定小鼠血清肝功能两项、小鼠肝匀浆MDA含量和SOD活性,通过HE染色后光镜观察小鼠肝脏病理变化。结果 与正常组相比,模型组的各项指标均有所改变,差异有显著统计学意义(P<0.01),肝脏病理切片显示损害明显;用药后与模型组比较,藏茵陈组ALT、AST及MDA含量均有所下降,超氧化物歧化酶(SOD)活性有所升高,各指标两组间差异均有统计学意义(P<0.01),而两药物组的肛温有回归正常范围趋势,但无统计学意义,藏茵陈组小鼠体重与模型组相比有所下降,差异有统计学意义(P=0.013);两药物组的肝脏病理切片结果显示均有所改善,以藏茵陈组较明显。结论 初步判断藏茵陈对于MHV-A59病毒感染湿热证的治疗主要通过抗病毒、抗脂质过氧化发挥作用,但用药后小鼠体重有所下降,可能与藏茵陈的苦寒导致脾胃功能损伤有关,可继续探索藏茵陈的最佳用药配伍及用药方式。
Objective To observe the effect of Tibetan Artemisiae capillaries on the mouse model of Damp-heat syndrome caused by MHV-A59 virus.Methods 32 mice were randomly divided into four groups： control group,model group,Virazole group and TAC group.After establishing the model,the serum contents of ALT,AST and the liver homogenate content of MDA and the activity of SOD were detected and the pathological changes of liver was observed through the light microscope after HE staining.Results Compared with control group,all of the indicators of model group showed the changes.Compared with model group,the contents of ALT,AST and MDA in TAC group were decreased,while the activity of SOD was increased.The pathology of TAC group was also better than that of model group.The improvement of rectal temperature could not be seen obviously,but the change of weight had an opposite effect.Conclusion Tibetan Artemisiae capillaries can improve the liver function and pathology and anti-virus and reducing lipid peroxidation are the main mechanisms of TAC on damp-heat syndrome infected by MHV-A59 virus.The weight-lowing effect of mice may be caused by the spleen dysfunction because of the bitter cold of TAC.So the best medication compatibility and the mode must be explored in the future.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第8期1822-1824,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金.广东省联合基金(No.U0632009)
关键词
藏茵陈
湿热证
MHV-A59
超氧化物歧化酶
Tibetan Artemisiae capillaries
Damp-heat syndrome
MHV-A59
Superoxide dismutase
