摘要
核异常作为组织特异性的遗传毒理体内短期检测法,现已日益受到重视。它比微核测试更敏感和合理。核异常包括多种形式的核损伤,而它们与致癌因子的损伤有着不同程度的相关性。本文以r-射线作为致癌与诱变因子,在人体外周血淋巴细胞中系统地比较研究了常用核损伤指标:微核、核变形、核碎裂和核固缩等的剂量(0-5Grag)一反应关系,并作线形回归分析。作者认为,作为人体淋巴细胞核异常测试法,应包括微核,核变形及核碎裂3个核损伤指标。
In order to use appropriately NAT and explore possibility of use of the test in human cells, the authors used γ-Tays as mutagenic and carcinogenic irradiated whole blood in vitro, prepared directly smears of isolated lymphocytes, and studied comparatively dose-response relationship of 4 types of nuclear damage. Main results of the study are given as follows:1. In doses from O to 3 Grays MNF (Frequency of micronucleus), INF (Frequency of irregular nucleus), KNF (Frequency of karyorrhetic nucleus), and PNF (Frequency of pykno-tic nucleus) increase along with increase of irradiated doses. At 5 grays INF still increases, but MNF, KNF and PNF decrease.2. In doses from O to 3 grays regression equation of 4 indices of nuclear damage are as follows (D means dose, r means correlative coefficient):MNF: y = 0.3433 + 0.10520, r = 0.9!28, p<0.05; INF: y=7.2178 + 2.1817D, r = 0.8846, p<0.05; KNF: y = 0.6462 + 0.20140, r = 0.7286, p>0.05; PNF: y = 0.2774 + 0.07280, r= 0.5484, p>0.05.3. In doses from O to 3 grays MNF is linear with INF and KNF. Regression equation are as follows (x means MNF):MNF-INF: y = 2.0598 + 16.6545x, r = 0.778 p>0.05;MNF-KNF: y = 0.2235+ 1.426x. r = 0.5945, p>0.05.Finally according to synthetical analysis of correlative coefficient, intercept, regression coefficient and feasibility of indices of nuclear damage, we could consider it suitable that nuclear anomalies in human peripheral lymphocytes include micronucleus, irregular nucleus and kary-orrhexis.
关键词
外周血
淋巴细胞
核损伤
NAT
Human peripheral blood Lymphocytes, Nuclear anomaly test, γ-ray
