摘要
目的研究8-羟基脱氧鸟苷(8-hydroxy-2-deoxyguanosine,8-OHdG)与宣威地区女性肺癌的关系。方法利用ELISA方法测定尿液中8-OHdG含量。采用饱和苦味酸法测定尿液中肌酐含量。最终数值采用尿肌酐(creafine,Cr)值较正,单位为ng/mg。结果 (1)8-OHdG在所有患者的尿液中均有表达,在肺癌患者尿液中含量高于肺良性病变患者(P<0.01);(2)宣威地区女性肺癌患者尿液中8-OHdG含量高于宣威地区男性肺癌患者和非宣威地区女性肺癌患者(P<0.01);(3)宣威地区女性肺良性病变患者尿液中8-OHdG含量高于宣威地区男性肺良性病变患者和非宣威地区女性肺良性病变患者(P<0.01)。结论 DNA氧化损伤产生的8-OHdG可能在肺癌的发生、发展中起着重要作用。宣威地区女性受到的氧化损伤高于宣威地区男性和非宣威地区女性,这可能是宣威地区女性肺癌高发的因素之一。
Objective To reveal the relationship between female lung cancer in Xuanwei region and 8-hydroxy- 2-deoxyguonosine（8-OHdG）. Methods 8-OHdG Content in urine was measured using the 8-OHdG ELISA （enzymelinked immunosorbent assay）. The urine creatinine was measured using picric acid. The final results of 8-OHdG content was expressed with ng/m_g creatinin; Results （1）8-OHdG were expressed in the urine samples of all patients, the content of 8-OHdG in urine of lung cancer patients was much higher than that of benign pulmonary diseases patients （P〈0.01）. （2）The content of 8-OHdG in urine of female lung cancer patients was higher than that of male lung cancer patients in Xuanwei region in Xuanwei region and female lung cancer patients in other regions（P〈0.01）. （3）The content of 80HdG in urine of female be- nign pulmonary diseases patients was much higher than that of male benign pulmonary diseases patients and female benign pulmonary diseases patients in other regions （P〈0.01）. Conclusion The 8-OHdG produced by DNA oxidative damage maybe play an important role in the occurrence and development of lung cancer. The oxidative damage of female in Xuanwei region is suffered higher than male in Xuanwei region and female in other regions. This may be one risk factor of high incidence of female lung cancer in other regions.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第10期1178-1180,共3页
Cancer Research on Prevention and Treatment
基金
云南省创新强省-国际合作专项基金资助项目(2008CA030)
