摘要
目的:研究喉鳞状细胞癌组织中信号转导和转录激活因子3(Stat3)及其靶基因细胞周期蛋白D1(Cyclin D1)的表达情况并探讨其临床意义。方法:应用Western blot和RT-PCR方法,研究喉鳞状细胞癌组织中Stat3、Cyclin D1蛋白和mRNA的表达情况以及与喉鳞状细胞癌临床病理因素的关系。结果:喉鳞状细胞癌组织中Stat3、Cyclin D1蛋白和mRNA的表达水平明显高于正常喉黏膜组织(P<0.01)。Stat3、Cyclin D1蛋白表达水平与喉鳞状细胞癌临床分期、分化程度及淋巴结转移有关(P<0.01);在喉鳞状细胞癌组织中Stat3蛋白、mRMA与CyclinD蛋白、mRNA的表达均呈正相关(r=0.564、0.552,均P<0.01)。结论:Stat3信号转导通路在喉鳞状细胞癌的发生、发展过程中起重要作用,检测喉鳞状细胞癌组织中Stat3及其靶基因Cyclin D1的表达可以反映肿瘤的恶性程度。
Objective：To examine the expression and clinical significance of the Stat3 and Cyclin D1 in laryngeal squamous cell carcinoma（LSCC）.Method：The expression of Stat3 and Cyclin D1 in LSCC tissue were detected by the RT-PCR and Western blot method respectively,and then the relationship between Stat3 and Cyclin D1 protein expression level and relevant clinical factors of LSCC was explored.Result：Compared to the normal larynx mucosa group,the expression level of Stat3 and Cyclin D1 was significantly higher in LSCC（P0.01）.The high expression level of Stat3 and Cyclin D1 protein in LSCC was correlated with the clinical stage,tumor differentiation and lymph node metastases（P0.01）.The expression level of Stat3 was positively correlated with that of Cyclin D1,The correlation coefficient（r） was 0.564（P0.01） for the protein expression and 0.552（P0.01）for the mRNA expression.Conclusion： Stat3 signaling pathway may play an important role in the tumorigenesis and progression of LSCC.The expression level of Stat3 and its target gene Cyclin D1 can reflect the malignancy degree of LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2011年第21期966-969,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
