摘要
目的探讨重组人甲状旁腺素（parathyroid hormone，PTH）对辐射所致小鼠造血功能损伤的防护作用。方法雄性C57小鼠60只，经^60Coγ射线照射后随机分为PTH治疗组和照射对照组。PTH治疗组每天给予PTH80μg·kg^-1·d^-1。腹腔注射，对照组给予等量生理盐水。全自动血细胞分析仪检测小鼠外周血细胞数，手工计数骨髓有核细胞数，流式细胞仪分析骨髓中CD34＋细胞数，集落细胞培养法观察骨髓粒系造血祖细胞数。结果在照射后10、15和20d，PTH治疗组小鼠的外周血红细胞计数显著高于照射对照组（t=6．48、3．66和4．98，P〈0．05）。在照射后各时间点外周血白细胞计数显著高于照射对照组（t=6．32、9．19、11．18、7．44和4．42，P〈0．05）。在照射后5和30d血小板计数显著高于照射对照组（t=2．57和3．10，P〈0．05）。在照射后各时间点PTH治疗组骨髓有核细胞数均显著高于照射对照组（t=5．80、3．72、17．89、4．90和16．49，P〈0．05）。PTH显著增加了照射后小鼠的骨髓粒细胞集落形成单位数和CD34＋细胞数（t=16．12、7．82和20．00，P〈0．05）。结论甲状旁腺素对辐射引起的造血损伤具有明显的保护作用。
Objective To study the protective effects of parathyroid hormone （PTH） on radiationinduced hematopoietie damage in mice. Methods A total of sixty male C57 mice were irradiated by ^60Coγ-rays to induce hematopoietic injuries, and then the mice were randomly divided into PTH and control group. The PTH-treated group was treated with PTH （ 80 μg·kg^-1·d^-1 ） intraperitoneally everyday. The control group was given equivalent volume saline. Peripheral blood cell number, bone marrow mononuclear cell number, granulocyte-macrophage colony forming units （CFU-GM） and CD34 positive cells in bone marrow were detected. Results With the whole post-irradiation period, the WBC and bone marrow mononuelear cell numbers in PTH-treated mice were significantly higher than those in saline-treated mice （t=6.32, 9.19, 11.18, 7.44 and 4.42,P 〈 0.05）. The RBC numbers in PTH-treated mice were significantly higher than those in control mice at 10 d, 15 d and 20 d post-irradiation （t = 6.48, 3.66 and 4.98, P 〈 0.05 ）. The PLT numbers in PTH-treated mice were significantly higher than those in control group at 5 and 30 d post-irradiation （ t = 2.57 and 3.10, P 〈 0.05）. PTH increased CD34 positive cell and CFU-GM numbers in bone marrow after irradiation （ t = 16. 12, 7.82 and 20. 00, P 〈 0.05 ）. Conclusions PTH could improve the hematopoietic recovery after irradiation.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2011年第5期542-544,共3页
Chinese Journal of Radiological Medicine and Protection
基金
江苏省自然科学基金（BK2009120）
关键词
甲状旁腺素
辐射损伤
造血功能
Parathyroid hormone
Radiation damage
Hematopoiesis
