摘要
采用ISSR分子标记技术,对浙江省桐庐县白云源森林公园的6个长叶榧(Torreya jackii)亚居群进行分析,阐明长叶榧在小地理范围内的遗传结构。12个引物对6个长叶榧亚居群的120个个体进行扩增,共得到194个位点,其中多态位点87个,总多态位点百分率(P)为44.85%,平均为29.21%。长叶榧总Shannon信息指数(I)为0.166 3,平均为0.119 9;总Nei指数(h)为0.1035,平均为0.075 5。P、I、h均表明长叶榧总体水平的遗传多样性较高,亚居群水平的遗传多样性较低。AMOVA分子变异分析显示,21.45%的变异存在于亚居群间,78.55%的变异存在于亚居群内。长叶榧亚居群间的遗传分化系数(Gst)为0.270 5,基因流为1.347 8。6个长叶榧亚居群的平均遗传距离为0.037 0,根据亚居群间的遗传距离进行UPGMA聚类,结果为生境相似的亚居群聚在一起。
Using inter-simple sequence repeat（ISSR） molecular technique,six subpopulations of Torreya jackii（Taxaceae） collected from Baiyunyuan Forest Park of Tonglu county of Zhejiang province were analyzed,the genetic structure of T.jackii was revealed in small scale.Using 12 primers,87 polymorphic loci from total 194 loci were detected in 120 individuals from 6 subpopulations and the overall percentage of polymorphic loci（P） was 44.85% with an average of 29.21%.Shannon’s information index（I） and Nei’s gene diversity（h） of T.jackii were 0.166 3 and 0.103 5 with an average of 0.119 9 and 0.075 5,respectively.P,I and h indicated that the genetic diversity at species level was relatively high,while at subpopulation level was relatively low.Analysis of molecular variation（AMOVA） suggested that the variation was 21.45% among subpopulations and 78.55% within subpopulation.The coefficient of gene differentiation（Gst） was 0.270 5 among subpopulations and the gene flow was 1.347 8.The mean genetic distance of six T.jackii subpopulations was 0.037 0.Based on the genetic distance,the clustered result for six T.jackii subpopulations by unweighted pair group method arithmetic average（UPGMA） suggested that the subpopulations in the similar habitat were clustered together.
出处
《植物研究》
CAS
CSCD
北大核心
2011年第6期722-728,共7页
Bulletin of Botanical Research
基金
浙江省自然科学基金项目(Y5090258)
华东师范大学博士研究生科研创新项目(CX2011013)
关键词
长叶榧
ISSR
亚居群
遗传多样性
遗传分化
Torreya jackii
ISSR
subpopulation
genetic diversity
genetic differentiation
