摘要
以青岛文昌鱼的晚期囊胚和早期原肠胚的胚胎细胞为实验材料,利用低渗处理、空气干燥法制备染色体玻片标本。在此基础上,为进一步探索显示青岛文昌鱼染色体G带的新方法,采用片龄、蛋白酶K和胰蛋白酶显带技术对青岛文昌鱼的染色体进行了初步研究,显示了青岛文昌鱼染色体的G类带型,绘制了青岛文昌鱼染色体的G类带型模式图,并对三种G类带型作了比较,进而提出了文昌鱼染色体的G带带型与片龄的相互关系。
The chromosomal patterns of amphioxus （Branchiostoma belcheri tsingtauens） were studied using blastula and early gastrula stages of embryos underwent hypotonic treatment and air-drying. New techniques for G-type banding were investigated using trypsin, protease K, and slide aging treatment. The G-type bandings of amphioxus were obtained and the chromosomal profiles were shown. The characteristics of the three G-type bands were compared, the similarities and differences were discussed. In addition, the correlation between the slide aging and G-banding was proposed.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2011年第4期597-602,共6页
Oceanologia Et Limnologia Sinica
基金
国家"863"项目资助
2008AA092604号
山东省教育厅项目资助
J08LE03号
江西省教育厅项目资助
GJJ11490号
关键词
青岛文昌鱼
染色体带型
G带
片龄
Amphioxus Branchiostoma belcheri tsingtauense, Chromosome banding, G-banding, Slide aging
