摘要
为加快番茄黄化曲叶病抗病品种培育进程,阻止该病蔓延,以抗病亲本CLN2777A和感病亲本TMXA48-4-0及其F2分离群体为材料,应用分子标记辅助选择技术进行抗TYLCV(番茄黄化曲叶病毒)基因连锁标记的筛选。通过对亲本及F2分离群体进行TYLCV接种,发现F2代抗感病分离比率接近3∶1,抗性受单个显性基因控制。用512对MseⅠ/PstⅠ引物组合对亲本及F2代抗感基因池进行AFLP分析,最终引物组合P15M28在亲本及抗感组间表现多态性,回收并克隆测序,片段大小为106 bp,重新设计引物,将其成功转化为CAPS(酶切扩增多态性序列)标记,命名为P15M28,遗传距离为6.7 cM。P15M28标记可以应用于标记辅助选择育种。
Tomato yellow leaf curl disease,caused by tomato yellow leaf curl virus（TYLCV） has become one of the major diseases of cultivated tomatoes throughout the world.In this study,a resistant parent CLN2777A,a susceptible parent TMXA48-4-0 and F2 progeny were used to screen a marker related to resistant gene for TYLCV.Through the inoculation of the parents and F2 progeny with TYLCV by whiteflies,it showed that segregation ratio of symptom conformed with the expected segregation of 3∶1 and the resistance to TYLCV was controlled by a single dominant gene.AFLP analysis was performed on parents and resistant/susceptible bulks using 512 pairs of MseⅠ/PstⅠprimers.The results showed that the primer combination P15/M28 was associated with the resistant gene.The fragment length was 106 bp,and it was converted to a cleaved amplified polymorphic sequence（CAPS） marker,named P15M28,successfully.The genetic distance of CAPS marker was 6.7 cM,it could be applied in marker-assistant selection breeding.
出处
《江苏农业学报》
CSCD
北大核心
2011年第5期1047-1052,共6页
Jiangsu Journal of Agricultural Sciences
基金
江苏省自主创新资金项目[CX(10)407]
公益性行业(农业)科研专项经费项目(201003065)
农业部"引进国际先进农业科学技术"项目(2010-Z35)
关键词
番茄黄化曲叶病毒
AFLP
CAPS
分子标记
tomato yellow leaf curl virus（TYLCV）
AFLP
cleaved amplified polymorphic sequence
molecular marker
