期刊文献+

GC法同时测定鸦胆子油中4种脂肪酸的含量 被引量:9

GC determination of four fatty acids in Brucea javanica(L.)Merr. oil simultaneously
原文传递
导出
摘要 目的建立同时测定鸦胆子油中棕榈酸、硬脂酸、油酸和亚油酸4种成分含量的方法。方法采用毛细管气相色谱法,甲酯化后测定鸦胆子油中棕榈酸、硬脂酸、油酸和亚油酸的含量。色谱柱:DB-17弹性石英毛细管柱(30 m×0.25 mm×0.25μm),检测器:氢火焰离子化检测器(FID),柱温恒温:200℃(保持11 min),进样口温度:250℃,检测器温度:250℃,流速:1.0 mL.min-1,分流比:20∶1,内标物:苯甲酸苯酯。结果棕榈酸、硬脂酸、油酸和亚油酸的线性分别为74~738 mg.L-1(r=0.999 6)、51~509 mg.L-1(r=0.999 1)、741~7 410 mg.L-1(r=0.999 1)和265~2 650 mg.L-1(r=0.999 1);各成分的平均回收率(n=9)分别为94.6%、94.2%、94.2%和95.3%。结论此方法可为鸦胆子油的质量评价提供依据。 Objective To establish a method for the determination of palmitic acid, octadecanoic acid, oleic acid and linoleic acid in Brucea javanica ( L. ) Merr. oil simultaneously. Methods The sample was determined with capillary column DB-17(30 m ×0. 25 mm x0. 25 μm) by GC coupled with flame ionization detector. Temperature was maintained at 200 ℃ for 11 rain. The injector temperature was 250℃. The detector temperature was 250 ℃. The flow rate was 1.0 mL.min-1. Split injection was conducted with split ratio of 20: 1. Nitrogen was used as carrier gas. Results The calibration curve was linear within the range of 74- 738 mg.L-1(r=0. 999 6) ,51-509 mg.L-1 (r =0. 999 1) ,741-7 410 mg.L-1(r=0. 999 1)and 265-2 650 mg. L - 1 ( r = 0. 999 1 ) for palmitic acid, octadecanoic acid, oleic acid and linoleic acid, respectively. The average recoveries (n = 3 )were 94. 6%, 94.2 % ,94. 2% and 95. 3 % for palmitic acid, octadecanoic acid, oleic acid and linoleic acid, respectively. Conclusions This method is convenient, accurate and reproducible for the determination of palmitic acid, octadecanoic acid, oleic acid and linoleic acid in Brucea javanica( L. ) Merr. oil.
出处 《沈阳药科大学学报》 CAS CSCD 北大核心 2011年第11期882-885,共4页 Journal of Shenyang Pharmaceutical University
基金 辽宁省教育厅科技专项课题(LT201001)
关键词 鸦胆子油 气相色谱法 脂肪酸 含量测定 Brucea javanica( L. ) Merr. oil GC fatty acid assay
  • 相关文献

参考文献4

二级参考文献15

  • 1丁怡,唐星.柱前衍生HPLC法测定鸦胆子油中的脂肪酸含量[J].中草药,2004,35(9):988-991. 被引量:21
  • 2孙苓苓,王淑丽.气相色谱法测定鸦胆子油乳制剂中油酸的含量[J].药物分析杂志,1996,16(2):98-100. 被引量:12
  • 3孙毓庆,1991年
  • 4袁佩英,山西中医,1990年,6卷,4期,24页
  • 5宋永春,食品工业科技,1990年,6卷,53页
  • 6孙毓庆,辽宁肿瘤防治研究,1983年,抗癌药鸦胆子专辑卷,17期,31页
  • 7许洪霞,沈阳医学院学报,1979年,11期,27页
  • 8ChP(中国药典).2000.VolⅠ(一部):341
  • 9Mehta A,Annette M.Oeser,et al.Rapid quantitation of free fatty acids in human plasma by high-performance liquid chromatography[J].J Chromatogr B,1998,719:9-23.
  • 10Ch P (中国药典)[S].Vol Ⅰ.2005.

共引文献35

同被引文献133

引证文献9

二级引证文献51

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部