摘要
以牡丹品种赵粉(Paeonia suffruticosa L.cv.Zhao Fen)为试材,采用RT-PCR和RACE法从花瓣中获得了1个牡丹SEPALLATA基因cDNA,命名为PsMADS5,GenBank登录号为HQ449569。其cDNA全长1222bp,包含190bp的5'非编码区、302bp的3'非编码区和1个长度为732bp编码243个氨基酸的开放阅读框。序列比对和系统进化分析表明,PsMADS5与葡萄的亲缘关系最近,相似性达83%以上,属于MADS家族SEP亚家族。相对荧光定量PCR分析表明,PsMADS5在花瓣中的表达量最高,其次是心皮,再次是萼片,在雄蕊中表达量最低。成功构建正义和反义表达载体,为牡丹花型改良和品种创新提供基础。
In this work,a full-length cDNA sequence of SEPALATAgene was obtained from petals of tree peony（Paeonia suffruticosa L.cv.Zhao Fen）using RT-PCR and RACE,named PsMADS5（GenBank accession No.HQ449569）.The full length of PsMADS5cDNA is 1222bp in length,containing a 5′-untranslated region（5′-UTR） of 190bp,a 3′-UTR of 302bp,and an opening reading frame（ORF） of 732bp encoding a 243 predicted amino acids.Subsequently,sequence comparison and phylogenetic analysis revealed that PsMADS5shared more than 83% homology with Vitis vinefera,belonged to SEPsubfamily of MADS family.However,relative real-time PCR analysis indicated that PsMADS5showed the highest transcript abundance in petals,moderate levels in carpels,low levels in sepals and the lowest levels in stamens.Construction of sense and antisense plant expression vector laid a foundation for flower type modification and variety innovation.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第5期939-944,共6页
Journal of Nuclear Agricultural Sciences
基金
国家863项目(2006AA100109)
国家林业局948项目(2006-4-C07)
关键词
牡丹
基因克隆
表达
载体构建
tree peony
gene cloning
expression
construction of vector
