摘要
以NC 89烟草(Nicotiana tabacum)为材料,通过农杆菌介导法获得转铜绿假单胞杆菌(Pseudomonas aerugi-nosa)KatB基因植株,利用PCR和RT-PCR对转化烟草进行鉴定,最后通过水分胁迫、盐胁迫、过氧化氢胁迫进行抗逆性分析。结果表明:与野生型植株相比,在缺水条件下的第13 d,300 mmol/L的NaCl胁迫条件下第20 d和0.3mmol/L的H2 O2胁迫条件下第33 d,转KatB基因烟草的叶片相对含水量、伤害率、过氧化氢酶(CAT)、丙二醛(MAD)、光系统Ⅱ(PSII)相对量子产率各种生理指标都显著优于野生型(WT)烟草,转KatB基因烟草表现出了较强的抗逆性,说明KatB基因在抗逆基因工程方面具有较好的应用前景。
NC 89 tobacco plants were transformed via Agrobacterium tumefaciens.PCR and RT-PCR indicate that the foreign gene KatB of Pseudomonas aeruginosa has been integrated into the genome of tobacco.Stress resistance tests were carried out under water stress,300 mmol/L NaCl stress and 0.3 mmol/L H2O2 stress.The results proved that the physiological indexes including leaf relative water content,MDA activity,relative conductivity and maximum photochemical yield of PSII of KatB tobacco were evidently higher than those of wild tobacco on the 13th day of water stress,20th day of 300 mmol/L NaCl stress and the 33rd day of 0.3 mmol/L H2O2 stress.The stress resistance of transgenic plants was high,which testified that there is a good application prospect of KatB gene in stress resistance of genetic engineering.
出处
《干旱区研究》
CSCD
北大核心
2011年第6期1064-1070,共7页
Arid Zone Research
基金
国家转基因专项(2008ZX080052004)
兵团博士基金(2006jc07)
