摘要
目的探讨尿肾损伤分子-l（Kjm-1）、尿N-乙酰-β-D-氨基葡萄糖苷酶（N—ace-cyl-β-D—glucosaminidase，NAG）及尿微量白蛋白（mALB）在重症感染中合并急性肾损伤的敏感性及临床价值。方法回顾分析60例在本院ICU住院的重症感染合并急性肾损伤（AKI）患者的尿Kim-l、尿NAG及尿mALB的变化情况。健康体检者20例为对照组。尿Kim-l、mALB测定采用酶联免疫法（ELISA）检测，尿NAG测定采用对硝基苯酚（PNP）比色法检测。结果AKI组患者尿液中的Kim-1、NAG、mALB的测定浓度明显高于对照组，差异具有统计学意义（P〈0．001），通过ROC曲线、诊断试验结果显示：尿Kim-1曲线下面积为0．955，95％可信区间为0．910-1．000，较尿NAG、mALB更具有敏感性（P〈0．001），两组问患者年龄及男女比例差异均无统计学意义（P〉0．05）。结论尿Kim-1对重症感染中合并急性肾损伤有诊断意义，检测尿Kim—l的浓度有助于急性肾损伤的早期诊断，对预防急性肾损伤的发生、发展具有重要的临床价值，
Objectives To explore the sensitivity and clinical value of urine kidney injury molecule - 1 （ Kim - 1 ） ,urine N - acetyl - beta - D - glucosaminidase（NAG） and urine microalbumin（mALB） in patients of severe infection with acute kidney injury. Methods To retrospectively analysis the changes of urine Kim - l, urine NAG and urine mALB in 60 cases of severe infection with acute kidney injury who hospitalized in ICU. 20 healthy subjects as control group. The urine Kim - 1 and urine mALB were respectively determined by enzyme linked immunosorbent assay （ ELISA ）. Urine NAG was measured by p - nitrophenol （PNP） colorimetric assay. Results Urine Kim - 1, urine NAG and urine mALBwere significantly higher in AKI group, compared with control group, there were statistically signifieanees between AKI group and control group （ p 〈 0. O01 ）. Through the ROC curve and diagnostic test ,the results showed that, the area under the curve of urine Kim - 1 was 0. 756, the 95% confidence interval was 0.910- 1.O00,urine Kimlwas more sensitive than urine NAG and urine mALB （ p 〈0.001 ） , No statistically significant difference was found in age,gender proportion between AKI group and control group（ p 〉 0.05 ）. Conclusions Urine Kiln - 1 had the diagnosis significance for patients of severe infection with acute kidney injury , detecting Urine Kim - 1 was helpful for the early diagnosis of acute kidney injury, it had important clinical value on the prevention in pathogenesis and development of acute kidney injury.
出处
《国际泌尿系统杂志》
2011年第6期730-732,共3页
International Journal of Urology and Nephrology
基金
新疆维吾尔自治区人民医院课题资助（项目编号：20100118）
