摘要
从紫叶小檗(Berberis thumbergii cv.atropurpurea)根、茎、叶和果实中分离纯化得到28株内生真菌,分别将其进行液体培养,培养物烘干研磨后用丙酮浸提其化学成分,采用琼脂扩散法分别测定各提取物对三种供试指示菌的抑菌活性.结果表明:有18株内生真菌丙酮粗提物至少对一株供试指示菌的抑菌圈直径大于10 mm,占所分离内生真菌菌株总数的64.3%;紫叶小檗各组织中均存在丰富的抗病原菌的内生真菌资源,菌株R1,S1,S7,L2,L4等5株内生真菌具有较广的抗菌谱.
28 endophytic fungi were isolated from the root,stem,leaf and fruit of Berberis thumbergii cv.Atropurpurea.The endophytic fungi were cultivated in liquid medium and the cultures were dried,grinded,and then extracted with acetone.The anti-bacterial activities of the acetone extracts on three species of bacteria were tested by agar diffusion method.The results showed that the acetone extracts of 18 strains of endophytic fungi exhibited an anti-bacterial circle（their diameter is more than 10 mm） against at least one indicator,and is of the total number of endophytic fungi 64.3%.The endophytic fungi with anti-bacterial activities in all tissues of Berberis thumbergii cr are resourceful and R1,S1,S7,L2 and L4 have a wider antibacterial spectrum.
出处
《鲁东大学学报（自然科学版）》
2011年第4期324-327,351,共5页
Journal of Ludong University:Natural Science Edition
关键词
紫叶小檗
内生真菌
抑菌活性
Berberis thumbergii cv. atropurpurea
endophytic fungi
antimicrobial activity
