摘要
将烯丙基缩水甘油醚(AGE)和β-环糊精在碱性条件下作用得到带有α烯基的环糊精衍生物——4-(3-烯丙氧-2-羟基)丙氧基-β-环糊精(PCD),利用这种衍生物和甲基丙烯酸甘油酯(GMA)为功能单体,在毛细管中通过原位聚合反应,一步法制备得到了新型β-环糊精聚合物毛细管电色谱手性整体柱。在毛细管电色谱(CEC)模式下,应用制备的手性整体柱对3种氨基酸和手性药物愈创甘油醚对映体进行拆分,并考察了有机调节剂、缓冲溶液pH值及柱温对拆分效果的影响。结果显示,在优化色谱条件下,4种对映体均得到基线分离,分析时间小于8 min。所制备的新型手性整体柱具有较强的手性拆分能力,且重现性好、操作简单。
In alkaline conditions, 4-（ 3-acryloxy-2-hydroxy ） propyloxy-β-cyclodextrin（ PCD ） was synthesized based on the reaction of 1-alklyloxy-2, 3-epoxy propane （also named as allyl glycidyl ether, AGE） and β-cyclodextrin（β-CD）. A new type β-cyclodextrin polymer chiral monolithic column for CEC was prepared by using the in-situ polymerization of PCD and GMA as functional monomers in the capillary tube. The prepared monolithic column was evaluated by separating racemic mixtures of three amino acids and a chial drug （guguaifenesin） in capillary electrochromatography mode. The influences of organic modifier, buffer pH value and temperature on separation were investigated. ibility and easy operation.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第11期1290-1294,共5页
Journal of Instrumental Analysis
