摘要
目的探讨大鼠血清中Th1/Th2细胞因子表达与供肝非实质细胞（NPC）输注诱导心脏移植免疫耐受的关系。方法建立大鼠心脏移植模型,将40只大鼠随机分成排斥反应组、免疫耐受组,每组20只。观察移植心脏存活时间,并检测受者血清中Th1/Th2细胞因子白细胞介素2（IL-2）、干扰素γ（IFN-γ）、白细胞介素4（IL-4）、白细胞介素6（IL-6）、白细胞介素10（IL-10）的表达水平。结果免疫耐受组供心存活时间为（30.83±2.55）d,排斥反应组为（8.15±2.08）d,差异有显著性（t=24.46,P〈0.01）。术后7、14 d排斥反应组血清Th1细胞因子IL-2、IFN-γ水平均高于免疫耐受组,Th2细胞因子IL-4、IL-10表达水平均低于免疫耐受组,术后7 d血清IL-6表达水平显著高于免疫耐受组（t=4.50~16.20,P〈0.01）。结论免疫耐受的形成与Th1/Th2细胞因子相互作用有关。
Objective To study the relationship between Th1/Th2 cytokine expression in serum and immune tolerance,induced by non-parenchymal cells（NPC） of donated liver,of cardiac allografts in rats. Methods A model of heart transplantation was created in 40 rats,and evenly randomized to rejection group and immune-tolerance group.The survival time of the implanted heart was observed,the expressions of serum Th1/Th2 cytokines： interferon-γ（IFN-γ）,interleukin-2（IL-2）,interleukin-4（IL-4）,interleukin-6（IL-6） and interleukin-10（IL-10）,were measured. Results The survival time of heart allografts in tolerance group was（30.83±2.55） d,and that in rejection group was（8.15±2.08） d,the difference being significant between the two groups（t=24.46,P0.01）.Seven and 14 days after transplantation,the expression of Th1 cytokines（IL-2,IFN-γ） were much higher in rejection group than in immune-tolerance group,and that of Th2 cytokines（IL-4,IL-10） were lower.Seven days after surgery,the expression of IL-6 in rejection group was much higher than immune-tolerance group（t=4.50-16.20,P0.01）.Conclusion The formation of immune tolerance is associated with interaction of Th1/Th2 cytokines.
出处
《青岛大学医学院学报》
CAS
2011年第5期399-401,405,共4页
Acta Academiae Medicinae Qingdao Universitatis
