摘要
目的观察消癌解毒方的抗肿瘤作用机理。方法采用人肝癌细胞SMMC-7721,经过细胞培养、MTT法、流式细胞仪Annexin V-FITC法检测消癌解毒方含药血清对肝癌细胞增殖和凋亡的影响。结果消癌解毒方具有良好的抑制肝癌细胞生长、促进凋亡的作用,尤其以高剂量组〔抑制率为35.8%,凋亡率为(23.0±1.6)%〕的效果最佳,作用程度与化疗药物顺铂〔抑制率为35.3%,凋亡率为(22.8±4.4)%〕相似(P>0.05)。结论消癌解毒方含药血清能抑制人肝癌细胞SMMC-7721生长,作用机制需要进一步的研究。
Objective To investigate the anticancer effect and mechanism of Xiao＇ai Jiedu Prescription（XJP）.Methods Human hepatoma SMMZ-7721 cells were cultured together with pharmacological serum of XJP.The effect of XJP on inhibiting the growth of SMMZ-7721 was evaluated by methyl thiazolyl tetrazolium（MTT） assay,and the apoptosis rate was detected with Annexin V-FITC by flowcytometry.Results It was shown that pharmacological serum of XJP inhibited the growth and promoted the apoptosis of human hepatoma SMMZ-7721 cells.The effect of large-dosage XJR group was best,with inhibition rate of 35.8 % and apoptotic rate of（23.0±1.6）%,which was similar to that of chemotherapeutics of cisplatin,with inhibition rate being 35.3 % and apoptotic rate being（22.8±4.4）%（P 〉0.05）.Conclusion Pharmacological serum of XJP could inhibit human hepatoma SMMZ-7721cells,and its mechanism needed further research.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2011年第6期587-589,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金项目(30973742/C190201)
江苏省高校自然科学研究面上项目(09KJB360003)
江苏省中医药局科技项目(LB09032)
关键词
消癌解毒方
含药血清
SMMC-7721
增殖
凋亡
Xiao＇ai Jiedu Prescription
Pharmacological Serum
Hepatoma SMMZ-7721cells
Proliferation
Apoptosis
